Inhibitory potential of some selected essential oils and their main components on the growth and quorum-sensing based pigment production of "Serratia marcescens"

Authors

  • Judit PAPP Babeș-Bolyai University, Hungarian Department of Biology and Ecology, Cluj-Napoca, Romania. judit.papp@ubbcluj.ro https://orcid.org/0000-0002-9192-9610
  • Maria IACOB Babeș-Bolyai University, Hungarian Department of Biology and Ecology, Cluj-Napoca, Romania; Corresponding author, E-mail: judit.papp@ubbcluj.ro

DOI:

https://doi.org/10.24193/subbbiol.2022.2.03

Keywords:

essential oils, quorum-sensing, prodigiosin.

Abstract

In this study the antibacterial potential of ten essential oils (EOs) and their main compounds against the development and quorum sensing (QS) mechanisms of the opportunistic bacterium Serratia marcescens was determined. The growth and QS inhibitory effect was evaluated by paper disc diffusion assay. The effect of EOs and components on QS-regulated prodigiosin biosynthesis was also studied. The results of our study indicated that some of the investigated EOs influenced the development and the QS-based activity of S. marcescens. Oregano and thyme oils showed the most pronounced antibacterial effect and had the strongest anti-QS potential. From the main oil compounds the phenolics, eugenol, carvacrol and thymol, proved to be efficient growth inhibitors. While eugenol and carvacrol had also a strong negative influence on AHL-mediated QS-systems in low concentrations, thymol was not effective in QS inhibition.

Article history: Received: 5 October 2022; Revised: 19 October 2022;
Accepted: 21 November 2022; Available online: 22 December 2022.

References

Annous, B.A., Fratamico, P.M. & Smith, J.L. (2009). Quorum sensing in biofilms: why bacteria behave the way they do. J. Food Sci., 74, 24-37.

Bakkiyaray, D., Sivasankar, C. & Pandian, S.K. (2012). Inhibition of quorum sensing regulated biofilm formation in Serratia marcescens causing nosocomial infections. Bioorg. Med. Chem. Lett., 22, 3089-3094.

Bakkali, F., Averbeck, S., Averbeck, D. & Idaomar, M. (2008). Biological effect of essential oils – a review. Food Chem. Toxicol., 46, 446-475.

Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods – a review. Internat. J. Food Microbiol., 94, 223-253.

Brown, S.P. & Johnstone, R.A. (2001). Cooperation in the dark: signaling and collective action in quorum-sensing bacteria. Proc. Biol. Sci., 268, 961–965.

Cosentino, S., Tuberoso, C.I.G., Pisano, B., Satta, M., Mascia, V., Arzedi, E. & Palmas, F. (1999). In vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Lett. Appl. Microbiol., 29, 130-135.

Coulthurst, S.J., Williamson, N.R., Harris, A.K.P., Spring, D.R. & Salmond, G.P.C. (2006). Metabolic and regulatory engineering of Serratia marcescens mimicking phage-mediated horizontal acquisition of antibiotic biosynthesis and quorum-sensing capacities. Microbiology, 152, 1899-1911.

de Kievit, T.R. & Iglewski, B.H. (2000). Bacterial quorum sensing in pathogenic relationships. Infect. Immun., 68, 4839-4849.

Do, H.N.A. & Nguyen, T.H.K. (2014). Studies on the prodigiosin production from Streptomyces coelicolor in liquid media using heated Lactobacillus rhamnosus. J. Applied Pharmaceut. Sci., 4, 21-24.

Dušan, F., Sabol, M., Domaracká, K. & Bujňáková, D. (2006). Essential oils – their antimicrobial activity against Escherichia coli and effect on intestinal cell viability. Toxicol. In Vitro, 20, 1435-1445.

Gill, A.O. & Holley, R.A. (2006). – Disruption of Escherichia coli, Listeria monocytogenes and Lactobacillus sakei cellular membranes by plant oil aromatics. Internat. J. Food Microbiol., 108, 1-9.

Haddix, P.L., Jones, S., Patel, P., Burnham, S., Knights, K., Powell, J.N. & LaForm, A. (2008). Kinetic analysis of growth rate, ATP, and pigmentation suggests an energy-spilling function for the pigment prodigiosin of Serratia marcescens. J. Bacteriol., 190, 7453-7463.

Kamble, K.D., Hiwarale, V.D. (2012). Prodigiosin production from Serratia marcescens strains obtained from farm soils. Internat. J. Environ. Sci., 3, 631-638.

Kerekes, E.B., Deák, É., Takó, M., Tserennadmid, R., Petrovits, T., Vágvölgyi, C. & Krisch, J. (2013). Anti-biofilm forming and anti-quorum sensing activity of selected essential oils and their main components on food-related micro-organism. J. Appl. Microbiol., 115, 933-942.

Khanafari, A., Assadi, M.M. & Fakhr, F.A. (2006). Review of prodigiosin, pigmentation in Serratia marcescens. J. Biol. Sci., 6(1), 1-13.

Kim, J., Marshall, M.R. & Wei, C.I. (1995.) Antibacterial activity of some essential oil components against five foodborne pathogens. J. Agricult. Food Chem., 43, 2839-2845.

Kumar, J.S., Umesha, S., Prasad, K.S. & Niranjana, P. (2016). Detection of quorum sensing molecules and biofilm formation in Ralstonia solanacearum. Curr. Microbiol., 72, 297-305.

Lis-Balchin, M. & Deans, S.G. (1997). Bioactivity of selected plant essential oils against Listeria monocytogenes. J. Appl. Microbiol., 82, 759-762.

Mekhael, R. & Yousif, S.Y. (2009). The role of red pigment produced by Serratia marcescens as antibacterial and plasmid curing agent. J. Duhok Univ., 12, 268-274.

Morohoshi, T., Shiono, T., Takidouchi, K., Kato, M., Kato, N., Kato, J. & Ikeda, T. (2007). Inhibition of quorum sensing in Serratia marcescens AS-1 by synthetic analogs of N-acyl-homoserine lactone. Appl. Environ. Microbiol., 73, 6339-6344.

Mourey, A. & Canillac, N. (2002). Anti-Listeria monocytogenes activity of essential oils components of conifers. Food Contrib., 13, 289-292.

Packiavathy, I.A.S.V., Agilandeswari, P., Musthafa, K.S., Pandian, S.K. & Ravi, A.V. (2012). Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolite methyl eugenol against Gram negative bacterial pathogens. Food Res. Internat., 45, 85-92.

Robson, E., Yan-Lue, L., Kok-Keng, T., Wai-Fong, Y. & Kok-Gan, C. (2014.) Quorum sensing activity of Serratia fonticola strain RB-25 isolated from an ex-landfill site. Sensors, 14, 5136-5146.

Samrot, A.V., Senthilkumar, C.K. & Kumar, N.G. (2011). Optimization of prodigiosin production by Serratia marcescens SU-10 and evaluation of its bioactivity. Internat. Res. J. Biotechnol., 2, 128-133.

Seydim, A.C. & Sarikus, G. (2006). Antimicrobial activity of whey protein based edible films incorporated with oregano, rosemary and garlic essential oils. Food Res. Internat., 39, 639-644.

Slater, H., Crow, M., Everson, L. & Salmond, G.P. (2003). Phosphate availability regulates biosynthesis of two antibiotics, prodigiosin and carbapenem, in Serratia via both quorum-sensing-dependent and -independent pathways. Molec. Microbiol., 47, 303–320.

Szabó, M.Á., Varga, G.Z., Hohmann, J. & Schelz, Z. (2010). Inhibition of quorum-sensing signals by essential oils. Phytother. Res., 786, 782–786.

Thomson, N.R., Crow, M.A., McGowan, S.J., Cox, A. & Salmond, G.P. (2000). Biosynthesis of carbapenem antibiotic and prodigiosin pigment in Serratia is under quorum sensing control. Molec. Microbiol., 36, 539-556.

Turgis, M., Vu, K.D., Dupont, C. & Lacroix, M. (2012). Combined antimicrobial effect of essential oils and bacteriocins against foodborne pathogens and food spoilage bacteria. Food Res. Internat., 48, 696-702.

Van Houdt, R., Givskov, M. & Michiels, C.W. (2007). Quorum sensing in Serratia. FEMS Microbiol. Rev., 31, 407-424.

Waters, C.M. & Bassler, B.L. (2005). Quorum sensing: cell-to-cell communication in bacteria. Annu. Rev. Cell Develop. Biol., 21, 319–346.

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Published

2022-12-22

How to Cite

PAPP, J., & IACOB, M. (2022). Inhibitory potential of some selected essential oils and their main components on the growth and quorum-sensing based pigment production of "Serratia marcescens". Studia Universitatis Babeș-Bolyai Biologia, 67(2), 35–49. https://doi.org/10.24193/subbbiol.2022.2.03

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