Expression of adhesins by some “Bordetella pertussis” strains isolated in Romania in different time periods

Authors

  • Georgeta Cristina OPREA University of Bucharest, Faculty of Biology. E-mail: crisstina_bio@yahoo.com
  • Veronica LAZĂR University of Bucharest, Faculty of Biology. Email: veronica.lazar2009@gmail.com  veronica.lazar@bio.unibuc.ro

DOI:

https://doi.org/10.24193/subbbiol.2018.2.05

Keywords:

adherence, fimbriae, pertussis vaccine, virulence factors.

Abstract

Bordetella pertussis is the etiological agent of whooping cough or pertussis, a respiratory infection in humans which can be prevented by vaccination. B. pertussis is characterized by a set of virulence factors involved in bacterial adherence to host-cells and consecutive colonization of the respiratory epithelium and in immunity too. In this study, we compared the expression of the specific adhesins with antigenic properties in some B. pertussis strains, including collection strains, vaccine strains and recently-isolated strains. The specific adhesins / surface antigens of B. pertussis are: a surface protein, named FHA (filamentous hemagglutinin) and fimbria Fim2 and Fim3. These antigens were evidenced using an indirect ELISA method, based on specific monoclonal antibodies binding to specific epitopes. The results showed that fimbrial antigens Fim3 are expressed by all new-isolated strains, compared to the older isolates, which expressed Fim2 or both Fim2, 3.

Author Biographies

Georgeta Cristina OPREA, University of Bucharest, Faculty of Biology. E-mail: crisstina_bio@yahoo.com

Cantacuzino National Institute of Medico-Military Research - Development, 103 Splaiul Independentei, sector 5, Bucharest, 050096. University of Bucharest, Faculty of Biology. E-mail: crisstina_bio@yahoo.com

Veronica LAZĂR, University of Bucharest, Faculty of Biology. Email: veronica.lazar2009@gmail.com  veronica.lazar@bio.unibuc.ro

University of Bucharest, Faculty of Biology. Email: veronica.lazar2009@gmail.com     veronica.lazar@bio.unibuc.ro

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Published

2018-12-17

How to Cite

OPREA, G. C., & LAZĂR, V. (2018). Expression of adhesins by some “Bordetella pertussis” strains isolated in Romania in different time periods. Studia Universitatis Babeș-Bolyai Biologia, 63(2), 73–80. https://doi.org/10.24193/subbbiol.2018.2.05

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