Bio-chemical analysis of “Datura stramonium” extract

Authors

  • Rahela CARPA Babeș-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.com https://orcid.org/0000-0001-8974-1718
  • Dana-Victoria DUMITRU Babeș-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania. Email: k_hella@yahoo.com
  • Ramona Flavia BURTESCU SC Plant Extrakt SRL, Radaia, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.com
  • Maria Cornelia MAIOR Babeș-Bolyai University, Institute of Technology, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.com
  • Cristina DOBROTĂ Babeș-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.com
  • Neli-Kinga OLAH Vasile Goldiș Western University of Arad, Faculty of Pharmacy, Arad, Romania. E-mail: k_hella@yahoo.com

DOI:

https://doi.org/10.24193/subbbiol.2017.2.01

Keywords:

atropine, bacterial susceptibility, Datura stramonium, High Performance Liquid Chromatography with reversed phase, scopolamine, thin-layer chromatography.

Abstract

Datura stramonium is an annual plant which belongs to the Solanaceae family. Datura stramonium is also a toxic plant, with toxicity given by the presence of alkaloids hyoscyamine, atropine and scopolamine.This study performs phytochemical and antibacterial analysis of ethanolic extracts from Datura stramonium. Fresh plants have been used in order to obtain Datura stramonium tincture. The quality index was 1:5, the concentration of ethanol being 90%. Based on this tincture a qualitative and quantitative phytochemical analysis was performed through thin layer chromatography and high performance liquid chromatography. By thin-layer chromatography the qualitative alkaloids, such as atropine and scopolamine from Datura stramonium extract, have been identified. By high performance liquid chromatography with reversed phase the alkaloids quantity from Datura stramonium herbs was assessed and that is 1.7 mg/mL. The plant extracts were tested on Gram negative bacteria Escherichia coli and on Gram positive bacteria Staphylococcus aureus. Both tested strains showed resistance but for E. coli a higher inhibition was observed at all samples containing Datura extract.

Author Biographies

Rahela CARPA, Babeș-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.com

Babeș-Bolyai University, Faculty of Biology and Geology, 1 M. Kogălniceanu Street, 400084, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.com

Laboratory of Plant Biology, Genetics, Microbiology and Biotechnology, Center of Systems Biology, Biodiversity and Bioresources, Babeş-Bolyai University, Cluj-Napoca, Romania.

Dana-Victoria DUMITRU, Babeș-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania. Email: k_hella@yahoo.com

Babeș-Bolyai University, Faculty of Biology and Geology, 1 M. Kogălniceanu Street, 400084, Cluj-Napoca, Romania. Email: k_hella@yahoo.com

Ramona Flavia BURTESCU, SC Plant Extrakt SRL, Radaia, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.com

SC Plant Extrakt SRL, 407059, Radaia, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.com

Maria Cornelia MAIOR, Babeș-Bolyai University, Institute of Technology, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.com

Babeș-Bolyai University, Institute of Technology, 30 Fantanele Street, 400294, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.com

Cristina DOBROTĂ, Babeș-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.com

Babeș-Bolyai University, Faculty of Biology and Geology, 1 M. Kogălniceanu Street, 400084, Cluj-Napoca, Romania.

Babeș-Bolyai University, Institute of Technology, 30 Fantanele Street, 400294, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.com

Neli-Kinga OLAH, Vasile Goldiș Western University of Arad, Faculty of Pharmacy, Arad, Romania. E-mail: k_hella@yahoo.com

SC Plant Extrakt SRL, 407059, Radaia, Cluj-Napoca, Romania.

Vasile Goldiș Western University of Arad, Faculty of Pharmacy, L.Rebreanu Street, Arad, Romania. E-mail: k_hella@yahoo.com

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Published

2017-12-20

How to Cite

CARPA, R., DUMITRU, D.-V., BURTESCU, R. F., MAIOR, M. C., DOBROTĂ, C., & OLAH, N.-K. (2017). Bio-chemical analysis of “Datura stramonium” extract. Studia Universitatis Babeș-Bolyai Biologia, 62(2), 5–19. https://doi.org/10.24193/subbbiol.2017.2.01

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