The effects of antibiotics and antifungals added to plant culture media of “Triticosecale wittmarck"
DOI:
https://doi.org/10.24193/subbbiol.2017.2.05Keywords:
: antibiotics, antifungals, culture media, Triticosecale.Abstract
The experiment aimed to study the antibiotics or antifungals effect to prevent the infection in Triticosecale wittmarck micropropagation, without affecting the inoculum. After identifying the pathogens, which are frequently affecting the in vitro culture media, respectively Clostridium sp. and Cladosporium sp., we made the antibiogram and antifungal susceptibility testing. Among antibiotics and antifungals, to which one of them were found to be sensitive, we chose to add the following to the culture media: V1-gentamicin (GEN) - 40 mg/L, V2-tetracycline (TET) - 10 mg/L, V3- ciprofloxacin (CIP) - 40 mg/L, V4- clotrimazole (CLO) - 50 mg/L, V5-fluconazole (FLU) - 50 mg/L, V6-terbinafine (TER) - 25 mg/L, having the culture medium, without anti-contaminants additions, as control lot. At the end of the experiment we concluded that anti-contaminants, used in concentrations recommended by literature, have prevented infections, but reduced the capacity for germination rate of triticale. Just terbinafine can be added in triticale culture media, but it is necessary to study other lower concentration that does not affect the growing of inoculum.
References
Arditti, J. (2008) Micropropagation of orchids, Second Edition, Vol. 1. Blackwell Publishing, pp. 90- 93
Barrett, C., Cassells, A.C. (1994) An evaluation of antibiotics for the elimination of Xanthomonas campestris pv.pelargonii (Brown) from Pelargonium x domesticum cv. ‘Grand Slam’ explants in vitro, Plant Cell, Tissue and Organ Culture, 36(2): 169-175
Brown, D.M., Groom, C.L., Cvitanik, M., Brown, M., Cooper, J.L., Arditti, J. (1982) Effects of fungicides and bactericides on orchid seed germination and shoot tip cultures in vitro, Plant Cell, Tissue and Organ Culture, 1(1): 165-180
Cachiță-Cosma, D., Ardelean, A. (2004) Tratat de biotehnologie vegetală, Vol. 1, Editura Dacia, pp. 20- 100
Cachiță-Cosma, D., Ardelean, A. (2009) Tratat de biotehnologie vegetală, Vol. 2. Editura Dacia, pp. 57- 170
Cercenado, E., Saavedra- Lozano, J. (2009): El antibiograma. Interpretación del antibiograma: conceptos generales (I), Anales de Pediatría Continuada, 7(4): 214-217
Cosma, A.D., Petruș-Vancea, A. (2017) Identificarea contaminanților din culturile in vitro vegetale, Științe Exacte și Stiinţe ale Naturii (Oradea), IX: 83-87
Costache, C., Colosi, I. (2008): Metoda difuzimetrică în testarea sensibilităţii de antifungice, Scientia Parasitologica, 1:74-78
Gamborg, O.L., Miller, R.A., Ojima, K. (1968) Nutrient requirement of suspensions cultures of soybean root cells., Exp. Cell Res., 50: 151-155
Guri, A.Z., Patel, K.N. (1998) Compositions and methods to prevent microbial contamination of plant tissue culture media. Patent US5750402 A [Accesed in 03.12.2017] https://www.google.ch/patents/US5750402
Kulkarni, A.A., Krishnamurthy, K.V. (2009): Contamination control and endhanced axillary budding from mature explant of Taxus baccata spp. Wallichiana. In: Plant tissue culture and molecular markers, Kumar, A., Shekhawt, N.S. (eds), pp. 1-29, I.K. International Publishing House PVT. LTD., New Delhi
Leonberger, K., Jackson, K., Smith, R., Gauthier, N. W. (2016) Plant diseases, Agriculture and natural resources publications, 46: 182
Petruș-Vancea, A., Cachiță-Cosma, D., Purcărea C. (2013) Proceduri bioeconomice și ecoeconomice de optimizare a conservării și multiplicării in vitro a biodiversității vegetale, Vasile Goldiș University Press, Arad, pp. 3-40
Makovitzki, A., Voterbo, A., Brotman, Y., Chet, I., Shai, Y. (2007) Inhibition of fungal and bacterial plant pathogens in vitro and in planta with ultrashort cationic lipopeptides, Applied Environmental Microbiology, 73(20): 6629–6636
Mocanu, M.M., Petruş-Vancea A. (2016) Abiotic antibiotic stress on triticale in vitro cultures, Natural Resources and Sustainable Development 2016: 100-107
Murashige, T., Skoog A. (1962) Revised medium for rapid growth and bioassays with tabbacco tissue cultures, Physiol. Plant, 15: 85-90
Opriș, O., Copaciu, F., Soran, M. L., Ristoiu, D., Niinemets, U., Copolovici, L. (2013) Influence of nine antibiotics on key secondary metabolites and physiological toxicity, Ecotoxicology and Environmental Safety, 87: 70-79
Smith, R.H. (2013) Plant Tissue Culture: Techniques and Experiments. Academic Press, pp. 155-158
Thomas, P. (2004) A three-step screening procedure for detection of covert and endophytic bacteria in plant tissue cultures, Current Science, 87: 67-72
Thurston, K.C., Spencer, S.J., Arditti, J. (1979) Phytotoxicity of fungicides and bactericides in orchid culture media, Am.J.Bot. 66: 825-835
Turcuș, V., Cachiță-Cosma, D. (2009) Drosera rotundifolia L. Particularități morfostructurale, Vasile Goldiș University Press, Arad, pp. 171- 176
Turdean, B.C., Petruş-Vancea, A. (2015) Efectul antibioticelor asupra germinației, Stiinţe Exacte și Stiinţe ale Naturii (Oradea), VII: 24-29
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