Theoretical and Biotechnological Approaches in the Institute of Biology Bucharest Between 1975 - 2015 based on Plant Cell and Tissue Culture Technology
Keywords:
electrostimulation, in vitro plant conservation, plant transformation, protoplasts, secondary metabolites in in vitro cultures.Abstract
In the present paper we highlight the main research stages of the laboratory of plant cell and tissue culture at the Institute of Biology, Bucharest from the moment of its beginning until present.
References
Aldea, F., Helepciuc, F.E., Banciu, C., Brezeanu, A., Soare, L.C. (2013) Comparative studies between ferns gametophyte and sporophyte by bidimensional electrophoresis, Muzeul Olteniei, Craiova, Studii şi comunicări, Ştiinţele Naturii, 29(1), 120-129
Anghel, I., Brezeanu, A., Toma, N. (1981) Ultrastructura celulei vegetale, Edit. Academiei R. S. R., pp. 206
Anghel, I., Vassu, T., Brezeanu, A. (1985) Aspecte citologice şi genetice ale izolării şi fuziunii protoplaştilor la unele tulpini de drojdii industriale, Al III-lea Simp. Naţ. Cult. Cel. Tes. Veg., Bucureşti, pp. 165-173
Anghel, I., Brezeanu, A., Vassu T. (1989a) Particular interactions between protoplasts and cells during the agglutination and fusion processes in yeasts, Rev. Roum. Biol.- Biol. Veget., 39(1), 13-31
Anghel, I., Vassu, T., Brezeanu, A. (1989b) Protoplast fusion for obtaining recombinants in industrial yeast, Rev. Roum. Biol - Biol. Veget., 34(2), 113-120
Angheluţă, R., Cialâcu, M., Badea, E. (1997) A histological examination of somatic embryogenesis in scutellar callus of wheat (Triticum aestivum L.), In: Proc. Inst. Biol., Falcă, M. (ed), Ed. Cultura, Piteşti, pp. 427-430
Antofie, M.M., Brezeanu, A. (2000a) Particularităţi ale multiplicării clonale in vitro la Spathiphyllum patinii, In: Actualităţi şi perspective în biotehnologiile vegetale, Lucr. IX Simp. Naţ. Cult. Ţes. Cel. Veget., Bavaru, A., Brezeanu, A., Cachiţă- Cosma, D. (eds), Universitatea "Ovidius" din Constanţa, Constanţa, pp. 135-151
Antofie, M.M., Brezeanu, A. (2000b) Analiza citofiziologică a calusului de Gardenia jasminoides privind efectul spermidinei asupra efluxului protonic indus de auxina naturală AIA, Acta Horti Bot. Bucurest., 28, 262-270
Antofie, M.M., Brezeanu, A. (2003) In vitro developmental peculiarities of Spathiphyllum, Aroideana (SUA), 26, 92-98
Antofie, M.M., Brezeanu, A. (2004) In vitro developmental peculiarities of Syngonium, Aroideana (SUA), 27, 172-181
Antofie, M., Carasan, M.E., Brezeanu, A. (1999) Total cellular peroxidase activity associated with wounding stress on Syngonium podophillum (Scott) in vitro tissues culture. In: Proc. Inst. Biol., Falcă, M. (ed), Ed. ALCRIS, Bucureşti, vol. II, pp. 339-343
Antofie, M.M, Brezeanu, A., Carasan, M.E., Maximilian, C. (2000) Morphological and biochemical aspects of acetylsalicylic acid influence on Gardenia jasminoides callus. In: Proc.Inst. Biol., Zarnea, G. and Falcă, M. (eds), Ed. Academiei Române, vol. III, 425-432
Antofie, M.M., Carasan, M.E., Brezeanu, A. (2003) Salicylic acid effects on two Gardenia jasminoides cell lines, Acta Horti Bot. Bucurest., 30, 135-145
Antofie, M.M., Carasan, M.E., Brezeanu, A. (2004) Petunia x hybrida as experimental model system for in vitro study in plant morphogenesis, Acta Horti Bot. Bucurest., 31, 161-166
Badea, E.M., Raicu, P. (1982) In vitro anther and pollen culture in haploids (n = 12) and triploids (3n = 36) of Datura innoxia Mill., Rev. Roum. Biol. - Biol. Veget., 27( 2), 163-166
Badea, E.M., Raicu, P. (1983) Experimental androgenesis in Datura innoxia plants with different levels of ploidy (2n, 4n, 6n), Rev. Roum. Biol. - Biol. Veget., 28(1), 59-61
Badea, E.M., Răduţoiu, S. (1999) The effect of hormones and salt stress on genome number variation in Datura innoxia androgenetic plants, In: Proc. Inst. Biol., vol. II, Falcă, M., Ed. Alcris, Bucureşti, pp. 287-289
Badea, E.M., Săndulescu, D. (2001) Biotehnologii vegetale, Fundaţia Biotech, Bucureşti, pp. 295
Badea, E.M., Scripcaru, A., Gregorian, L., Raicu, P. (1982) Studies on the tissue cultures differentiation in grapevine, Rev. Roum. Biol. - Biol. Veget., 27(1), 65-68
Badea, E.M., Iordan, M., Mihalea, A. (1985) Induction of androgenesis in anther culture of Digitalis lanata, Rev. Roum. Biol. - Biol. Veget., 30(1), 63-72
Badea, E.M., Papa, D., Lupuşanschi, I., Verzea, M. (1991) Study of regenerative potential and chromosomal variation in a long term culture of Triticum aestivum, Probl. Genet. Teor. Apl., 3-4, 123-131
Badea, E.M., Lupuşanschi, I., Verzea, M., Raicu, P. (1994a) Studiul unor factori implicaţi în variabilitatea citogenetică a plantelor cu origine polinică, Cercet. Genet. Veget. Anim., III, 71-78
Badea, E.M., Varga, P., Gregorian, L., Holobiuc, I. (1994b) In vitro selection for salt and aluminium tolerance in alfalafa (Medicago sativa L.), Proc. Conf. Eucarpia/ Medicago, pp. 238-241
Badea, E.M., Cialîcu, M., Holobiuc, I., Gregorian, L., Scripcariu, A. (1999a) Utilizarea variaţiei somaclonale pentru ameliorarea toleranţei la salinitate la plantele cultivate, In: Actualitati şi Perspective în Biotehnologiile Vegetale, Lucr. al 9-lea Simp. Naţ. Cult. Ţes. Cel. Veget., Bavaru, A., Brezeanu, A., Cachiţă- Cosma, D. (eds), Universitatea "Ovidius" din Constanţa, Constanţa, pp. 28-36
Badea, E.M., Gozia, O., Holobiuc, I., Gregorian, L., Gorenflot, R. (1999b) Obtention de somaclones de Medicago sativa L. tolerants au sel obtenus par selection in vitro dans des suspensions cellulaires, Rev. Biol. Cytol., 22 (3/4), 19-24
Badea, E.M., Holobiuc, I., Gozia, O., Cialîcu, M. (1999c) Expression of chimeric gene Phsp 70: GUS in transgenic Nicotiana tabacum, In: Proc. Inst. Biol., II, Falcă, M. (ed), Ed. Alcris, Bucureşti, pp. 283-286
Badea, E.M., Gozia, O., Holobiuc, I., Scripcariu, A., Brezuica, L. (2000) Studiul expresiei genei himere pHSP 70 (LP 19): GUS in embriogeneza la Datura innoxia Mill., L. Cerc. Genet. Veg. Anim., VI, 251-257
Banciu, C., Cristian, D. (2015) Cryoconservation of Pseudevernia furfuracea L. species and assessing the viability after thawing, Muzeul Olteniei Craiova. Oltenia. Studii şi comunicări. Ştiinţele Naturii, 31(1), 57-60
Banciu, C., Carasan, M.E., Brezeanu, A. (2009a) In vitro propagation of the endangered species Marsilea quadrifolia L. - morphological and biochemical analysis of the regenerates, Rom. Biotech. Lett., 14(1), 4139-4145
Banciu, C., Mitoi, M.E., Brezeanu, A. (2009b) Biochemical peculiarities of in vitro morphogenesis under conservation strategy of Ruscus aculeatus L., Ann. For. Res., 52, 109-116
Banciu, C., Mitoi, M., Helepciuc, F., Aldea, F. (2010) In vitro propagation of critically endangered species Scilla autumnalis L. - biochemical analyses of the regenerants, Analele Universităţii din Oradea - Fascicula Biologie Tom. XVII, 2, 318-323
Bavaru, A., Brezeanu, A., Doroftei, E. (2000) Cultura in vitro a algelor marine pluricelulare verzi: Ulva rigida, Cladophora vagabunda, In: Actualităţi şi perspective în biotehnologiile vegetale, Lucr. IX Simp. Nat. Cult. Tes Cel. Veget., Bavaru, A., Brezeanu, A., Cachiţă- Cosma, D. (eds), Universitatea "Ovidius" din Constanţa, Constanţa, pp. 135-151
Blîndu, R, Holobiuc, I. (2005) The evaluation of in vitro tuberization potential in three Romanian potato cultivars, In: Proc. Inst. Biol., VII, Zarnea, G, Dumitru, L. (eds), Ed. Academiei Romȃne, Bucureşti, pp. 275-282
Blȋndu, R., Holobiuc, I. (2006) Armeria maritima ssp. alpina - ex situ conservation using in vitro techniques, Acta universitatis cibiniensis, Agricultural Sciences, 1(1), 36-43
Blȋndu, R.D., Mitoi, E.M., Enache, M. (2010) Studii privind înființarea unei bănci de gene la specii vegetale de interes conservativ și biotehnologic din zona Retezat - Ţara Hategului, In: Conservarea Geo- şi Biodiversităţii şi dezvoltarea Durabilă în Ţara Haţegului - Retezat, vol.1, Otiman P.I. (ed), Ed. Academiei Române, București, pp. 417-422
Brezeanu, A. (1980) Callus initiation and morphogenesis induction in somatic tissue culture of Helianthus annuus L., Trav. Mus. Hist. Nat.”Grigore Antipa”, XXII, 37-41
Brezeanu, A. (1983) Ultrastructure of Helianthus annuus L. callus during the growth, differentiation and aging processes, Rev. Roum. Biol.- Biol. Veget., 28(1), 29-36
Brezeanu, A. (2009) Stresul - factor major în instalarea morţii celulare (MC) la plante, In: Adaptarea la Stres: Condiţie de Supravieţuire şi/sau Factor de Biodiversitate, Enache, M. and Dumitru, L. (eds), Ed. Academiei Romȃne, pp. 137-152
Brezeanu, A., Banciu, C. (2009) Comparative studies regarding ultrastructure of Marsilea quadrifolia L. (Pteridophyta) leaf mesophyll cells in vivo and in vitro culture, Rom. J. Biol. - Plant Biol., 54(1), 13-25
Brezeanu, A., Cogălniceanu, G. (2005) Cytodifferentiation and morphogenesis in Ocimum basilicum callus culture, Acta Horti Bot. Bucurest., 32, 109-113
Brezeanu, A., Davis, D.G. (1979) Succesive changes in the ultrastructure of Oryza sativa L. cells during growth and aging, Protoplasma, 98, 163-176
Brezeanu, A., Roşu, A. (1984) Isolation and culture of cell protoplasts from the mesophyll callus of Vitis vinifera L., Rev. Roum. Biol. - Biol.Veget., 29(1), 33-38
Brezeanu, A., Voicu, D. (2008) Ultrastructural peculiarities of the Usnea barbata (L.) Mott. mycobiont and phycobiont cells in vivo and in vitro cultures, Rom. J. Biol.- Plant Biol., 53(2), 63-71
Brezeanu, A., Iordan, M., Roşu, A. (1980) The micropropagation of Vitis vinifera L. I. The induction and characterization of callus from tissues of somatic origin, Rev. Roum. Biol. -Biol.Veget., 25(2), 135-142
Brezeanu, A., Iordan, M., Roşu, A. (1982a) Protoplast isolation by enzymatic procedures from Nicotiana tabacum (L.), Daucus carota subsp. safiuus (Hoffm.) Arcangcli, Vitis vinifera (L.) and Citrullus lanatus (Thumb.) Mansf., Rev. Roum. Biol. - Biol. Veget., 27(2), 133-140
Brezeanu, A., Iordan, M., Roşu, A., Mirancea, D., Popescu, I. (1982b) Cercetări privind iniţierea culturilor celulare şi inducerea morfogenezei in vitro in scopul multiplicării clonale la Vitis vinifera L., Probl. Genet. Teoret. Aplic., XIV(1), 43-76
Brezeanu, A., Matienco, B., Cogălniceanu, G., Călin, A., Cârciumărescu, D. (1993a) Iniţierea şi caracterizarea calusului din fruct şi rahis la Vitis vinifera L., In: Lucr. V-lea Simp. Naţ. Cult. Ţes. Cel. Veg., Anghel, I., Brezeanu, A., Cachiţă-Cosma, D. (eds), Edit. Universităţii Bucureşti, Bucureşti, pp. 181-191
Brezeanu, A., Petcu, I., Cogălniceanu, G., Radu, M. (1993b) Electromanipulări celulare – aplicaţii biotehnologice, In: Lucr.V-lea Simp. Naţ.Cult. Ţes. Cel.Veg., Anghel, I., Brezeanu, A., Cachiţă-Cosma, D. (eds), Edit. Universităţii Bucureşti, Bucureşti, pp. 109-122
Brezeanu, A., Banu, E., Pop, I., Coman, I. (1994a) Regeneration of vine mozaic virus-free Vitis vinifera L. plants using meristem culture and in vitro law temperature treatment, In: Proc. 8th Nat. Simp. Industr. Microbiol. Biotechnol., Anghel, I. (ed), Edit. Universităţii Bucureşti, Bucureşti, pp. 418-424
Brezeanu, A., Pătraşcu-Călin, A., Cornea, C.P., Vătafu, I. (1994b) Studii privind capacitatea transformantă a unor tulpini de Agrobacterium tumefaciens asupra ţesuturilor somatice de viţă de vie, Analele ICVV, XIII, 255-264
Brezeanu, A., Fologea, D., Cogălniceanu, G., Vătafu, I., Radu, M. (1999a) The expression of gus gene in N. tabacum cv. Xanthi electroporated intact seedlings using pJIT-79, In: Proc. Inst. Biol. Buc., vol. II, Falcă, M. (ed), Alcris, Bucureşti, pp. 273-281
Brezeanu, A., Matienco, B., Cogălniceanu, G., Maximova, E., Lupşea, S. (1999b) Hypothetical model of intracellular anthocyanins traffickingpathways in grape - vine long term callus culture, In: Proc. Inst. Biol., vol. II, Falcă, M. (ed), Alcris, Bucureşti, pp. 263-272
Brezeanu, A., Carasan, M.E., Drumitrescu, R., (2000) Efect of exogenous administration of spermidine on cell proliferation and morphogenesis procceses in in vitro cultures of N. tabacum (cv. Xanthi), In: Proc. Inst. Biol., vol. III, Zarnea, G., Falcă, M. (eds), Edit. Academiei Române, Bucureşti, pp. 375-383
Brezeanu, A., Antofie, M.M., Cogălniceanu, G. (2001) Ţesutul tumoral de tip crown gall un model experimental pentru studiul apoptozei la plante, In: Probleme Curente de Biologie Celulară şi Moleculară, vol. VI, Ardelean, A., Crăciun, C. (eds), Risoprint, Cluj-Napoca, pp. 53-68
Brezeanu, A., Antofie, M.M., Maximilian, C., Carasan, M., Zamfir, M., Cogălniceanu, G. (2002) Apoptoza, proces esenţial al dezvoltării plantelor, An. Soc. Nat. Biol. Cel. Molec., VII, Crăciun, C., Ardelean, A. (eds), Risoprint, Cluj-Napoca, pp. 40-52
Brezeanu, A., Cogălniceanu, G., Mihai, R. (2008) Ultrastructural characterization of the in vitro gametophyte of Bucegia romanica Radian - a rare liverworth, Rom. J. Biol.- Plant Biol., 53(2), 49-63
Brezeanu, A., Cogălniceanu, G., Mihai, R. (2009) Studying cell biology of bryophytes, Biotechnol. & Biotechnol. Eq., 23, 467-468
Carasan, M.E. (2009) Strategii de răspuns la stresul oxidativ determinat de deficitul hidric la plante, In: Adaptarea la stress, condiţie de supravieţuire şi /sau factor de biodiversitate, Enache, M., Dumitru, L. (eds), Edit. Academiei Române, Bucureşti, pp. 153-165
Carasan, M.E., Antofie, M. M, (2007) Variation of some biochemical parameters during indirect somatic embryogenesis in Petunia x hybrida, Acta Horti Bot. Bucurest., 34, 22-28
Carasan, M.E., Brezeanu, A. (2002) Efectul spermidinei administrată exogen asupra proliferării şi morfogenezei în culturi de peţiol de N. tabacum L. cv Xanthi. In: Lucr. X-lea Simp. Nat. Cult. Tes. Cel. Veget. “25 de ani de Culturi de Tesuturi Vegetale în România”, Cachiţă-Cosma, D., Rakosy-Tican, L., Ardelean, A. (eds), Risoprint, Cluj-Napoca, pp. 261-270
Carasan, M.E., Antofie, M.M., Brezeanu, A. (2003) The study of some oxidative processes induced by the osmotic shock on callus cells of Nicotiana tabacum cv Xanthi. In: Proc. Inst. Biol., vol.V, Zarnea, G., Falcă, M. (eds), Edit. Academiei Române, Bucureşti, pp. 459-464
Carasan, M.E., Antofie, M.M., Brezeanu, A. (2004a) Izoenzime markeri ai unor procese morfogenetice in vitro la plante, In:Lucr. Al XII-lea Simp. Nat. Cult. Tes. Cel. Veg., Fiziologia celulei vegetale în regim de vitrocultură, Cachiţă-Cosma, D., Ardelean, A., Fati, V. (eds), Ed. Daya, Satu-Mare, pp. 143-162
Carasan, M.E., Antofie, M.M., Maximilian, C.R., Zamfir, M., Brezeanu, A., (2004b) The evaluation of role of some antioxidant enzymes in response to abscisic acid and high osmoticum, In: Proc. Inst. Biol., vol. VI, Zarnea G. and Falcă M. (eds), Edit. Academiei Române, Bucureşti, pp. 371-380
Carasan, M.E., Antofie, M.M., Brezeanu, A. (2005) Particularităţi ale morfogenezei in vitro la Nicotiana tabacum cv Xanthi în condiţiile deshidratării celulare induse de PEG şi ABA, In: Al XIII-lea Simp. Nat. Cult. Tes. Cel. Veg., Vitroculturile la cormofite, modele experimentale în cercetările de biologie, Cachita-Cosma, D., Ardelean, A. (eds), Ed. Bion, Satu-Mare, pp. 28-35
Catană, R., Holobiuc I. (2015) Direct somatic embryogenesis of the endemic taxon Papaver alpinum L. ssp. corona-sancti-stefani (Zapal.) Borza for conservative purpose, Muzeul Oltenia. Studii şi comunicări. Ştiinţele Naturii, 31(1), 47-51
Catană, R., Ciucă, M., Holobiuc, I. (2010a) Using RAPD techniques to check the genetic stability of Erigeron nanus Schur. regenerants in the ex situ conservation context, Analele Universităţii din Oradea Fascicula Biologie, XVII(2), 230-234
Catană, R., Mitoi, M., Helepciuc, F., Holobiuc, I. (2010b) In vitro conservation under slow growth conditions of two rare plant species from Caryophyllaceae family, Electron. J. Biol., 6(4), 86-91
Catană, R., Mitoi, M., Ion, R. (2013) The RAPD techniques used to assess the genetic diversity in Draba dorneri, a critically endangered plant species, Adv. Biosci. Biotech., 4(2), 164- 169
Călin, A., Brezeanu, A. (1997) Potenţialul morfogenetic in vitro la Stevia rebaudiana (Bert.) Bertoni, In: Actualităţi şi Perspective în Biotehnologiile Vegetale, Cachiţă, D., Ardelean, A., Crăciun, C. (eds), Ed. Vasile Goldiş, Arad, pp. 147-151
Călin, A., Cucu, N., Tessio, C. (1996) Stability of a transgene in potato depends on endogenous plant tissue factors, Biotechnol. & Biotechnol. Eq., 10(2-3), 18-25
Cogălniceanu, D., Cogălniceanu, G.C. (2010) An enlarged European Union challenges priority settings in conservation, Biodivers. Conserv., 19(5), 1471-1483
Cogălniceanu, G. (2006) Electrical control of plant morphogenesis, In: Plant Tissue Culture Engineering, Series: Focus on Biotechnology, vol. 6, Dutta Gupta, S., Yasuomi Ibaraki (eds), Springer, the Netherlands, pp. 395-415
Cogălniceanu, G. (2014) Romanian in vitro bryophyte collection and its role for conservation, Acta Horti Bot. Bucurest., 41(1), 5-11
Cogălniceanu, G., Brezeanu, A. (1995) Developmental electrono-microscopy of exogenous progesterone influences on direct somatic embryogenesis from leaf and cotyledon explant of Nicotiana tabacum L. cv. Xanthi, Rev. Roum. Biol. - Biol. Veget., 40(1), 33-43
Cogălniceanu, G., Brezeanu, A. (2000) Stress factors and developmental homoeostasis in tobacco in vitro systems, In: Proc. Inst. Biol. Vol. III, Zarnea, G., Falcă, M. (eds), Edit. Academiei Române, Bucureşti, pp. 405-414
Cogălniceanu, G., Stoiculescu, R. (2007) Particularităţi privind culturile in vitro la briofite, In: Lucr. XV-lea Simp. Naţ. Cult. Ţes. Cel. Veg., Micropropagarea speciilor vegetale, Cachiţă-Cosma, D. (ed), Risoprint, Cluj-Napoca, pp. 97-106
Cogălniceanu, G., Cornea, P., Sântauan, M., Brezeanu, A. (1998a) Cell proliferation, regeneration capacity and peroxidase isoenzyme spectrum in fresh and long-term tobacco callus, Rom. Biotech. Lett., 3(1), 15-21
Cogălniceanu, G., Radu, M., Fologea, D., Brezeanu, A. (1998b) Are the electric field effects coupled with the hormonal reception of cells in plant cell culture?, Rom. Biotech. Lett., 3(3), 201-206
Cogălniceanu, G., Radu, M., Fologea, D., Moisoi, N., Brezeanu, A. (1998c) Stimulation of tobacco shoot regeneration by alternating weak electric field, Bioelectrochem. Bioenerg., 44, 257-260
Cogălniceanu, G., Brezeanu, A., Matienco, B., Lupşea, S. (2000a) Factori implicaţi în stimularera proliferării celulare şi a metabolismului secundar (biosinteza de antociani) în cultura de calus long - term la V. vinifera., Acta Horti Bot. Bucurest., 28, 282-291
Cogălniceanu, G., Radu M., Brezeanu A., Fologea D. (2000b) High voltage short duration pulses promote adventive shoot differentiation from intact tobacco seedlings, Electro. Magnetobiol., 19(2), 177-187
Cogălniceanu, G., Brezeanu, A., Roșu, A. (2001a) In vitro reactivity of somatic explants from Prosopis juliflora (Swartz) DC somatic explants, Acta Horti Bot. Bucurest., 29, 257-262
Cogălniceanu, G., Carasan, M., Radu, M., Fologea, D., Brezeanu, A. (2001b) The influence of external electric field on the in vitro postcotyledonary development of Nicotiana tabacum L. cv. Xanthi seedlings, Rom. Biotech. Lett,, 5(5), 45-54
Cogălniceanu, G., Mitoi, M., Helepciuc, F., Matei, M., Matei, S. (2010) Biochemical changes induced in regenerants of Fragaria X Ananassa Duch. by the in vitro treatment with fungal elicitors, Rom. Biotech. Lett., 15(4), 5512-5518
Cornea, C.P., Cogălniceanu, G., Brezeanu, A., Vătafu, I., Savu, L., Pop, A. (1992) Obţinerea protoplaştilor de tutun prin utilizarea unui preparat enzimatic brut de Streptomyces sp. SD16, St. Cercet. Biol.- Biol. Veget., 44(1-2), 85-88
Cornea, C.P., Vătafu, I., Pop, A., Brezeanu, A., Savu, L., Dobrotă, S. (1993) Interspecific protoplast fusion in Streptomyces, Rev. Roum. Biol.- Biol. Veget., 38(1), 49-53
Cristian, D., Mitoi, M., Brezeanu, A. (2013) In vitro systems, a facile tool for Cetraria islandica (L.)(Lichenophyta) artificial resynthesis and biotechnological potential exploit - preliminary results, Rom. J. Biol. - Plant Biol., 58(2), 95-104
Davis, D.G., Shimabukuro, R., Brezeanu, A. (1976) The effect of MPP on the ultrastructure of wheat in vivo and in vitro, Weed Sci., 289-325
Davis, D.G., Brezeanu, A., Shimabukuro, R. (1979) An ultrastructural study of Triticum monococcum cell suspension culture during aging and after treatment with herbicide Diclofop-methyl, Can. J. Bot., 98, 163-176
Dumitrescu, R. (2005) The ultrastructure changes produced by ABA+PEG and PEG alternative stress upon tobacco regenerant cells, In: Proc. Inst. Biol., vol. VII, Zarnea, G., Dumitru, L. (eds), Edit. Academiei Romȃne, Bucureşti, pp. 283-292
Dumitrescu, R., Carasan, M.E. (2002) Efectul spermidinei asupra conţinutului în pigmenţi asimilatori în calusul de N. tabacum L.cv Xanthi. In: Current Problems in Cellular and Molecular Biology, vol. VI, Crăciun, C., Ardelean, A.(eds), Risoprint, Cluj-Napoca, pp. 462-467
Dumitrescu, R., Carasan, E. M. (2006) Reactivity of tobacco regenerant cells to ABA and PEG (6000) alternative stress, Acta Horti Bot. Bucurest., 33, 29-34
Dumitrescu, R., Holobiuc I. (2005) Efectul indus de sorbitol asupra suspensiilor celulare de Nicotiana tabacum L. cv. Petite Havana transformate genetic, In: An. Soc. Nat. Biol. Cel., IX, Ardelean, A., Crăciun, C. (eds), Risoprint, Cluj- Napoca, pp. 335-344
Fologea, D., Brezeanu, A., Radu, M., Cornea, C.P., Vătafu, I. (1998) Gene transfer by electroporation into intact tobacco petiol tissue, Electro. Magnetobiol., 18(1), 1-6
Heplepciuc, F.E., Carasan, E. M., Brezeanu, A., Cornea, C.P., Voaideş, C. (2008) The evaluation of stimulative and protective effect induced by treatments with biocontrol agents on plant development, In: „Proceedings of the International Symposium on New Researches in Biotechnology”, Serie F, Special Volume: Biotechnology, pp. 562-568
Helepciuc, F.E., Mitoi, M.E., Păunescu-Manole, A., Aldea, F., Brezeanu, A., Cornea, C.P. (2014) Induction of plant antioxidant system by interaction with beneficial and/or pathogenic microorganisms, Rom. Biotech. Lett., 19(3), 9366-9375
Holobiuc, I., (2007) Stadiul conservării ex situ a plantelor cu statut periclitat pe baza utilizării tehnicilor in vitro, In: Biodiversitatea, de la concepte fundamentale la aplicații biotehnologice, Enache, M., Dumitru, L. (eds.), Edit. Academiei Romȃne, Bucureşti, pp. 150-168
Holobiuc, I. (2015) Somatic embryogenesis in long-term cultures of Gentiana lutea L. in the presence of osmotic stress, In: The Gentianaceae, Vol. 2: Biotehnology and Applications, Rybczynski, J. J., A. Mikula, M. R. Davey (eds.), Springer-Verlag Berlin Heidelberg, pp. 139-161
Holobiuc, I., Blîndu, R. (2006) Improvement of the micropropagation and in vitro medium-term preservation of some rare Dianthus species, Contributii Botanice Cluj, 41(2), 143-151
Holobiuc, I., Blȋndu R. (2006-2007) In vitro culture introduction for ex situ conservation of some rare plant species, Rom.J. Biol. - Plant Biol., 51-52, 13-23
Holobiuc I., Blîndu R. (2007) Micropropagarea în vederea conservării speciei rare cu potenţial ornamental Primula halleri J. F. Gmelin, In: Lucr. XV-lea Simp. Naţ. Cult. Ţes. Cel. Veg., Micropropagarea speciilor vegetale, Cachiţă-Cosma, D. (ed), Risoprint, Cluj-Napoca, pp. 107-116
Holobiuc, I., Catană, R. (2012) Recurrent somatic embryogenesis in long-term cultures of Gentiana lutea L. as a source for synthetic seed production for medium-term preservation, Arch. Biol. Sci, Belgrade, 64(2), 817-825
Holobiuc, I., Gozia, O., Badea, E. (1999) In vitro selection efficiency for high levels of NaCl in callus culture of Medicago sativa L., In: Proc. Inst. Biol., vol. II, Alcris, Bucureşti, pp. 301-308.
Holobiuc, I., Păunescu, A., Blîndu, R. (2004) Researches concerning micropropagation of the endemic species Hieracium pojoritense Wol., In: Proc. Inst. Biol., vol. VI., Zarnea, G., Falca, M. (eds), edit. Academiei Romȃne, Bucureşti, pp. 405-413
Holobiuc, I., Păunescu, A., Blȋndu, R. (2005) Ex situ conservation using in vitro methods in some Caryophyllaceae plant species from the Red List of the vascular plants in Romania, Rom. J. Biol.- Plant Biol., 49-50, 3-16
Holobiuc, I., Păunescu, A., Blîndu, R. (2005) Utilizarea culturilor in vitro pentru conservarea speciei endemice periclitate Astragalus pseudopurpureus Gusul., In: Al XIII-lea Simp. Naţ. Cult. Ţes. Cel. Veg., Vitroculturile la cormofite, modele experimentale in cercetarile de biologie, Cachiţă-Cosma, D., Ardelean, A. (eds), Bion, Satu Mare, pp. 104-114
Holobiuc, I., Voichita, C., Blîndu, R., Negrean, G.. (2006) In vitro conservation of the rare plant Veronica multifida L. ssp. capsellicarpa, Contribuţii Botanice Cluj, 41(2), 135-141
Holobiuc, I., Blîndu, R., Brezeanu, A. (2008a) Ex situ conservation in Gentiana lutea L. through somatic embryogenesis, In: Proc.Sec. Intern. Symp.“New researches in Biotechnology”, Series F, Special Vol. Biotechnology, Bucharest., pp. 449-458
Holobiuc, I., Blȋndu, R., Carasan, M., Helepciuc, F., Voichiţă, C.R., Negrean, G. (2008b) In vitro conservation strategy of Veronica multifida spp capsellicarpa (Dubrovik) A. Jelen, Rom. J. Biol. - Plant Biol., 53(2), 71-81
Holobiuc, I., Brezeanu, A., Blȋndu, R. (2009a) Somatic embryogenesis induction in presence of moderate osmotic stress, synthetic seeds production in rare Dianthus species from Romania Flora as tool for ex situ conservation, In: Proc. Sec. Intern.l Symp.“New researches in Biotehnology”, Series F, Special Vol. Biotechnology, Bucharest, pp. 69-77
Holobiuc, I., Blîndu, R., Cristea, V. (2009b) Researches concerning in vitro conservation of the rare plant species Dianthus nardiformis Janka, Biotechnol. & Biotechnol. Eq., 23(2), 221-224
Holobiuc, I., Blîndu, R., Mitoi, M., Helepciuc, F., Cristea, V. (2009c) The establishment of an in vitro gene bank in Dianthus spiculifolius Schur and Dianthus glacialis ssp. gelidus (Schott Nym. Et Kotschy) Tutin: I. The initiation of a tissue collection of the cultures in minimal growth conditions, Ann. For. Res., 52, 14-26
Holobiuc, I., Mitoi, M., Blȋndu, R., Helepciuc, F. (2010) The establishment of an in vitro gene bank in Dianthus spiculifolius Schur. and D. glacialis ssp. gelidus (Schott Nym. et Kotschy) Tutin: II. Medium-term cultures characterization in minimal growth conditions. Rom. Biotech. Lett., 15(2), 5111-5119
Holobiuc, I., Voichiţă, C., Catană, R., Mitoi, M., Helepciuc, F. (2014) Medium-term preservation of Dianthus trifasciculatus Kit ssp. parviflorus through minimal cultures, Oltenia. Studii şi Comunicări. Ştiinţele Naturii., 30(1), 57-66
Holobiuc, I., Voichiţă, C., Catană, R. (2015) In vitro conservation of the critically endangered taxon Convolvulus persicus L. and regenerants evaluation, Oltenia. Studii şi comunicări. Ştiinţele Naturii, 31(2), 51-59
Hristea, G., Cogălniceanu, G., Brezeanu, A. (2005) Carbonic material and obtaining procedure for anthocyanin pigment biosynthesis, Patent No. A / 00848 / 06.10.2005.
Iordan, M., Brezeanu, A. (1985) Comportarea unor clone de Salix în procesul de micropropagare in vitro, In: Lucr. al III-lea Simp. Naţ. Cult. Ţes. Cel. Veg., Anghel, I. (ed), Edit. Universităţii Bucureşti, Bucureşti, pp. 131-143
Iordan M., Brezeanu A., Roşu A. (1981a) The micropropagation of Vitis vinifera L. II. Aspects of morphogenesis in callus culture, Rev. Roum. Biol. - Biol. Veget., 26(2), 141-150
Iordan, M., Enescu, V., Mirancea, D., Roşu, A., Grigorescu, A. Brezeanu, A. (1981b) Iniţierea culturilor celulare şi inducerea morfogenezei in vitro la arbori (Quercus robur), In: Culturi de Ţesuturi şi Celule, Instrument de Cercetare în Biologia Teoretică şi Aplicativă, Tipogr. Agronomia, Cluj-Napoca, pp. 113-140
Iordan, M., Benea, V., Brezeanu, A., Coman, I. (1984) Multiplicarea clonală prin cultură de meristeme a arborilor - PopulusX Euramericana cv. Robusta R-16, Rev. Pădurilor, 2, 120-127
Iordan-Costache, M., Pătraşcu-Călin, A., Brezeanu, A., Cornea, C.P. (1996) Transfer de gene la genotipuri de plop (Populus) prin utilizarea de tulpini sălbatice şi modificate genetic de Agrobacterium tumefaciens, Cercetări de genetică vegetală şi animală, vol. IV, pp. 239-248
Lupşea, S., Brezeanu, A. (1999) Reactivitatea in vitro a sp. Vitis vinifera L. în funcţie de genotip, natura explantului şi mediul de cultură, Acta Horti Bot. Bucurest., 27, 87-92
Manole, A., Banciu, C. (2014) Conservarea in vitro a resurselor genetice a fitotaxonului steoendemic Campanula romanica Savul., In: Lucrările Conferinţei Internaţionale Genetica, Fiziologia şi Ameliorarea Plantelor, Print-Caro, Chişinău, pp. 268-271
Manole, A., Banciu, C. (2015) Optimization of shoot multiplication in Ruscus aculeatus L. from long term cultures, Rom. Biotech. Lett., 20(2), 10200-10204
Manole-Aiftimie, A., Mihai, R., Banciu, C. (2013) In vitro clonal propagation and whole plant regeneration for Serratula bulgarica Achtaroff et Stoj. germplasm conservation, Bul. Acad. Şt. Mold.- Ştiinţele vieţii, 3(321), 60-67
Manole-Păunescu, A. (2014) Biotechnology for endangered plant conservation, In: Biodiversity and Biotechnology, Ahuja, M. R. & Ramawat, K. G. (eds.), Springer International Publishing, Switzerland, pp. 181-202
Matienco, B., Brezeanu, A., Maximova, E., Marinescu, M., Cogălniceanu, G. (2004) Carpoculture in vitro. Nonmorphogenetic pathway, Î.E.P. Ştiinţa, Chişinău, pp 136
Maximilian, C., Brezeanu, A. (2004) Enzimes involved in oxidative processes associated with experimental apoptosis induced by infection with virulent Agrobacterium tumefaciens strains, In: Proc. Inst. Biol., vol. VI, Zarnea, G., Falcă, M. (eds), Edit. Academiei Romȃne, Bucureşti, pp. 425-433
Maximilian, C., Carasan, M. E (1999) Phosphatases activity in mycorhizal root from Zea mays. In: Current Problems in Cellular and Molecular Biology, vol. IV, Crăciun, C., Ardelean, A. (eds), Risoprint, Cluj-Napoca, pp. 576-579
Maximilian, C., Roşu, A., Brezeanu, A. (1998) Photonic and electronomicroscopically research on associations between plant cell - mycorrhizal fungi, Current Problems in Cell and Molecular Biology, vol. III, Crăciun, C., Ardelean, A. (eds), Risoprint, Cluj-Napoca, pp. 460-466
Maximilian, C., Carasan, M.E., Brezeanu, A. (2000) Identification of several enzymes involved in the colonization with mycorrhizal fungi in Zea mays plants, Proc. Inst. Biol., vol. III, Zarnea, G., Falcă, M. (eds), Edit. Academiei Române, Bucureşti, pp. 445-451
Maximilian, C., Carasan, M.E., Brezeanu, A., Roşu, A. (2001) Comparative analysis for detection of the ability of mycorrhizas establishment using species of Glomus, Current Problems in Cell and Molecular Biology, vol. V, Crăciun, C., Ardelean, A. (eds), Risoprint, Cluj-Napoca, 441-447
Maximilian, C., Brezeanu, A., Carasan, M.E., Roşu, A. (2002) Particularităţi ale interacţiei celulă vegetală - celulă fungică de micorize, In: Progrese în Biotehnologie, vol. 2, Dumitru, I. F. (ed), Edit. Ars Docendi, Bucureşti, pp. 197-217
Maximilian, C., Brezeanu, A., Carasan, M. (2003) Biochemical aspects associated with apoptosis in ‘crown gall’ tumor tissue. In: Proc. Inst. Biol., vol.,V, Zarnea, G., Falcă, M. (eds), Edit. Academiei Române, pp. 513-525
Matei, S., Matei, G.M., Cornea, P., Popa, G., Cogălniceanu, G., Drăghici, E., Bădulescu, L., Murariu, R., Georgescu, M., Săvulescu, E. (2011) Utilizarea experimentală a elicitorilor fungici pentru imunizarea plantelor contra putregaiului cenuşiu, Sitech, Craiova, pp. 150
Matei, A., Cornea, C.P., Matei, S., Matei, G.M., Cogălniceanu, G., Rodino, S. (2015) Biosynthesis of silver nanoparticles using culture filtrates of lactic acid bacteria and analysis of antifungal activity, Dig. J. Nanomater. Bios., 10(4), 1201-1207
Mihai, R., Brezeanu, A., Cogălniceanu, G. (2009) Aspects of some elicitors influence on non-morphogenetic callus of Vitis vinifera L. var. Isabelle, Rom. Biotech. Lett., 14(4), 4511-4518
Mihai, R., Mitoi, M., Brezeanu, A., Cogălniceanu, G. (2010) Two-stage system, a possible strategy for the enhancement of anthocyanin biosynthesis in a long-term grape callus cultures, Rom. Biotech. Lett., 15(1), 5025-5033
Mihai, R., Mitoi, M., Manole, A., Brezeanu, A. (2013) A preliminary study on the dynamics of total phenol and anthocyanin accumulation in Vitis vinifera L. cv. Negru Vârtos callus culture, J. Acad. Sci. Mold. (Life Sci.), 3(321), 68-72
Mirancea, D., Brezeanu, A. (1986) Morphogenesis in vitro in some Nicotiana species according to the natue of explant, hormonal balance and type of culture, Rev. Roum. Biol. – Biol. Veget., 31(1), 33-40
Mitoi, E.M., Blându, R.D. (2010) Evaluarea variabilităţii genetice la Draba dorneri Heuff. - specie endemică de interes conservativ în zona Retezat - Ţara Hategului, In: Conservarea Geo- şi Biodiversităţii şi dezvoltarea Durabilă în Ţara Haţegului-Retezat, vol.1, Otiman, P. I. (ed), Edit. Academiei Române, București, pp. 411-416
Mitoi, E.M., Holobiuc, I., Blîndu, R. (2009) The effect of mannitol on antioxidative enzymes in vitro long term cultures of Dianthus tenuifolius and Dianthus spiculifolius, Rom. J. Biol. - Plant Biol., 54(1), 25 - 33
Mitoi, M.E., Hristea, G., Cogălniceanu, G. (2013) Rapid and green synthesis of silver nanoparticles by strawberry callus extract, In: 18th Romanian International Conference on Chemistry and Chemical Engineering, Papers and Abstracts, Politehnica Press, Bucureşti, pp. S6-35
Panciu, I., Holobiuc I., Catană, R., (2014) The use of biotechnology for supplying of plant material for traditional culture of medicinal, rare species Arnica montana L., Lucr.i Şt.- Agr., Univ. Şt. Agr. Med. Vet., “Ion Ionescu de la Brad”, 57(1), 183-188
Pătraşcu A. (1981) Regeneration of potato plants by in vitro culture of stem segments, Rev. Roum. Biol. - Biol. Veget., 26(2), 151- 156
Pătraşcu, A., Brezeanu, A. (1989) Aspecte metodologice privind obţinerea şi purificarea protoplaştilor la cartof (Solanum tuberosum L.), Cercet. Genet. Veget. Anim., 1, 199-209
Pătraşcu, A., Cojocaru, N., Cateli, T (1985) Culturi de meristeme la două linii de cartof în scopul eradicării virusului S, In: Lucr. III-lea Simp. Nat. Cult. Cel. Tes. Veg., Anghel, I. (ed), Edit. Universităţii Bucureşti, Bucureşti, pp. 367-375
Pătraşcu-Călin, A., Cornea, C.P., Vătafu, I., Brezeanu, A. (1991) Preliminary results regarding transformation of several species of higher plants by Agrobacterium tumefaciens in in vitro explant culture, In: Simp. Nat. Cult. Cel. Tes. Veg., Cachiţă Cosma, D (ed), Risoprint, Cluj-Napoca, pp. 83-91
Păunescu, A., (2005) Conservation of the endemic plant Cerastium transsilvanicum Schur. of Piatra Craiului Massif using in vitro techniques, In: Researches in Piatra Craiului National Park, Phonix, Braşov, 3, pp. 99-103
Păunescu, A. (2007) Researches upon morphogenetic responses to vitroculture conditions of the phytoendemic taxa Alyssum borzaeanum Nyar., In: Al XVI-lea Simp.Naţ.Cult. Ţes. Cel. Veg., Cachiţă-Cosma, D. (ed), Risoprint, Cluj-Napoca, pp. 76-85
Păunescu, A. (2008) Biotehnologii de Conservare a Plantelor, Edit. Princeps, Iaşi, pp 158
Păunescu, A. (2009) Biotechnology for endangered plant conservation: a critical overview, Rom. Biotech. Lett., 14 (1), 4095-4103
Păunescu, A.(2010) In vitro propagation of Campanula polymorpha Witas. - an endemic plant of Carpathian Mountains, An. Univ Oradea. Fasc. Biol., 17(2), 235-238
Păunescu, A., Holobiuc, I. (2003) Conservation of the endemic species Dianthus callizonus Schott&Kotschy using in vitro techniques, Rev. Roum. Biol.- Biol.Végét., 48(1-2), 3-7
Păunescu, A., Holobiuc, I. (2005) Preliminary researches concerning micropropagation of some endemic plants from Romanian Flora, Acta Horti Bot. Bucurest., 32, 103-108
Păunescu, A., Vântu, S. (2002) Micropropagation of the endemic species Andryala levitomentosa (E. Nyar.) Sell., Rev. Roum. Biol.- Biol.Végét., 47(1), 9-11
Raicu, P., Badea, E.M. (1979) Direct and indirect androgenesis in Petunia hybrid, Rev. Roum. Biol. - Biol. Veget., 24(2), 171-174
Radu, M., Cogălniceanu, G., Brezeanu, A. (1994) Control of N. Tabacum L. callus growth by alterning and pulsed electric field, Electro. Magnetobiol., 13(3), 195-201
Roşu, A., Anghel, I. (1985) Studii asupra embriogenezei somatice la Solanum melongena, In: Lucr. III-lea Simp. Nat. Cult. Cel. Tes. Veg., Anghel, I. (ed), Edit. Universităţii Bucureşti, Bucureşti, pp. 399-409
Roşu, A., Anghel, I., Brezeanu, A. (1987) Aspects of in vitro cell differentiation in watermelon (Citrullus lanatus (THUNB) Mansf.), Rev. Roum. Biol. - Biol. Veget., 32(2), 97-110
Stoiculescu, R., Cogălniceanu, G., Brezeanu, A., Hristea, G. (2009) Influence of microstructurated carbon materials on Sequoia sempervirens (D. Don.) Endl. in vitro culture, Ann. For. Res., 52, 137-142
Verzea, M., Badea, E., Lupuşanschi, I., Moraru, I., Răducanu, F., Coman, I. (1994) Efectul genotipurilor parentale asupra capacităţii androgenetice la hibrizii F1 de Triticale hexaploid, Cercet. Genet. Veg. Anim., III, 55-91
Voichiţă, C., Brezeanu, A. (2005) in vitro a speciei periclitate şi rare Ecbalium elaterium L., In: Al XIV-lea Simp.Naţ. Cult. Ţes.Cel.Veg., Conservarea vitroculturilor vegetale, Cachiţă-Cosma, D., Sand, C. (eds), Alma Mater, Sibiu, pp. 77-86
Voichiţă, C., Helepciuc, F., Holobiuc, I. (2013) The use of isozymes markers for the characterization of Convolvulus persicus L. individuals belonging from Sulina population, Muzeul Olteniei Craiova. Oltenia. Studii şi comunicări. Ştiinţele Naturii., 29(2) 53-57
Voichiţă, C., Helepciuc, F., Holobiuc, I. (2014) Intrapopulational variability of endangered species Centaurea pontica Prodan & Nyar using isozymes electrophoretic spectra, Muzeul Olteniei Craiova. Oltenia. Studii şi comunicări. Ştiinţele Naturii, 30(1), 61-65
Voicu, D., Brezeanu, A. (2008a) In vitro reactivity of Usnea barbata (L.) Mott., Rom. J. Biol. Plant Biol., 53(2), 71-83
Voicu, D., Brezeanu, A. (2008b) Comportamentul în cultură in vitro a speciilor lichenice Xanthoria parietina (L.) Th. Fr. şi Cetraria islandica Ach., In: Al XVI-lea Simp.Naţ. Cult. Ţes.Cel.Veg., Biotehnologii vegetale pentru secolul XXI, Cachiţă-Cosma, D., Brezeanu, A., Ardelean, A. (eds), Risoprint, Cluj-Napoca, pp. 95-101
Zarnea, G., Avram, D., Vătafu, I., Brezeanu, A., Cornea, C.P. (1988) Protoplasts isolation, fusion and regeneration in bacteria of g. Bacillus, Rev. Roum. Biol.- Biol. Veget., 33 (1), 51-56
*** (1984) Protoplaştii, Tipografia Universităţii din Bucureşti, pp. 97
*** (2011) Conservarea Diversităţii Pteridofitelor din Valea Vâlsanului, Soare, L. C.(ed), Edit. Universităţii din Piteşti, pp. 214
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2016 Studia Universitatis Babeș-Bolyai Biologia
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
