Arsenite-induced activation of JNK1/2 and p38 MAP kinases in ELM-1 murine erythroleukemia cells
Keywords:
arsenite, erythroleukemia cells, JNK1/2, MAPK, p38.Abstract
Arsenite stimulates different mitogen-activated protein kinase (MAPK) pathways in the murine erythroleukemia cell line ELM-I-1. The activation of JNK1/2 and p38 was determined by Western blotting with antibodies specific for phosphorylated, activated forms of these kinases. Our data indicate that arsenite stimulates rapid phosphorylation of JNK1/2 and p38 MAPKs in ELM-I-1 cells. In the concentration range of 12.5-500 μM arsenite stimulates the stress response kinases JNK1/2 and p38. Maximal JNK1/2 and p38 activation was observed at 50 μM. This concentration produces an 7-40 fold increase in the activity of the JNK1/2, and an 4.5-7.5 fold increase in the activity of the p38 MAPK. The arsenite effects were time dependent: maximal activation of the stress response kinases JNK1/2 and p38 were observed after a 60 min exposure to arsenite.
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