Challenges in the detection and identification of potato virus Y, an important pathogen of potato
Keywords:
bioassay, ELISA, molecular detection, PVYᴺᵀᴺ, PVYᴺ⁻ᵂᶦAbstract
Potato virus Y (PVY) has become one of the most important pathogens of potato. Being a RNA virus, one of the main characteristic is it’s great genetic variability. Several well characterized strains du exist but the continuous emergence of new forms, the fast spreading of the existent ones and the shifting toward the increased prevalence of the recombinant necrotic strains raised a huge interest in finding ways for reducing the propagation of the disease. Two important strategies were adopted aiming for the same outcome, the reduction of PVY incidence. The first one, consists in improving the genetic background of potato and creating cultivars resistnt to PVY. This objective may be achieved by the integration of PVY resistance genes into potato gene pool. The second one, and this will be the subject of this review, relies on the finding of suitable methods for PVY detection which has to be fast and economical competitive for being applied to a large scale. The seed certification represents the most important step for multiannual and/ or interregional or international PVY spread prevention. There is no tolerance for necrotic PVY strains in seed, especially in the case of those batches that are obtained by biotechnology, and therefore, the screening for PVY became part of the protocol for potato seed certification.
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