Investigation of Methicillin-Resistant “Staphylococcus aureus” strains from Satu Mare using Molecular Biology Techniques

Authors

  • Evelyn-Erzsébet GABRI Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania. E-mail: eviergabri@gmail.com
  • Endre JAKAB Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania. E-mail: eviergabri@gmail.com https://orcid.org/0000-0002-3100-7083

Keywords:

mecA, MRSA, nucA, PCR, Staphylococcus aureus.

Abstract

Staphylococcus aureus s a clinically important bacteria. It causes mild to severe, often life-threatening infections. A total of 51 Staphylococcus strains were analyzed in this study using polymerase chain reaction. 33%f the strains were confirmed as S. aureus, and none of these were resistant to methicillin.

Author Biographies

Evelyn-Erzsébet GABRI, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania. E-mail: eviergabri@gmail.com

Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania. E-mail: eviergabri@gmail.com

Endre JAKAB, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania. E-mail: eviergabri@gmail.com

Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania. E-mail: eviergabri@gmail.com

References

Ito, T., Ma, X. X., Takeuchi, F., Okuma, K., Yuzawa, H., Hiramatsu, K. (2004) Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC, Antimicrobial Agents and Chemotherapy, 48(7), 2637-2651

Ito, T., Katayama, Y., Asada, K., Mori, N., Tsutsumimoto, K., Tiensasitorn, C., Hiramatsu, K. (2001) Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus, Antimicrobial Agents and Chemotherapy, 45(5), 1323-1336

Ito, T., Katayama, Y., Hiramatsu, K. (1999) Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315, Antimicrobial Agents and Chemotherapy, 43(6), 1449-1458

Matthews, P., Tomasz, A. (1990) Insertional inactivation of the mec gene in a transposon mutant of a methicillin-resistant clinical isolate of Staphylococcus aureus, Antimicrobial Agents and Chemotherapy, 34(9), 1777-1779

Song, M. D., Wachi, M., Doi, M., Ishino, F., Matsuhashi, M. (1987) Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion, FEBS Letters, 221(1), 167-171

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Published

2015-12-31

How to Cite

GABRI, E.-E., & JAKAB, E. (2015). Investigation of Methicillin-Resistant “Staphylococcus aureus” strains from Satu Mare using Molecular Biology Techniques. Studia Universitatis Babeș-Bolyai Biologia, 60(Sp.Issue), 29–32. Retrieved from http://193.231.18.162/index.php/subbbiologia/article/view/4621