The risk for colorectal cancer and polymorphisms of APC gene
Keywords:
Adenomatous polyposis coli, colorectal cancer, polymorphisms.Abstract
Colorectal cancer (CRC) is a complex genetic disease which appears as a result of interactions between multiple genetic and non-genetic factors. In this study we have analyzed the association between four polymorphisms of APC gene (rs41116, rs465899, rs2229992 and rs2019720) and colorectal cancer in Romanian population. After receiving the informed consent, blood samples were obtained from individuals with CRC (M:F=95:85) and from healthy persons (M:F=27:33). Genomic DNA was extracted from peripheral blood samples and the APC gene polymorphisms were assessed by PCR-RFLP. The promoter polymorphism rs2019720 was associated with CRC. Thus, the CC genotype (OR 2.307) and allele C (OR 1.843) increased the disease risk, while the AA genotype (OR 0.453) and allele A (OR 0.543) decreased the CRC risk. We found that the APC polymorphism rs2019720 was associated with CRC in Romanian population.
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