Preliminary data regarding genetic diversity of several endangered and endemic “Dianthus” species from Romania generated by RAPD markers

Authors

DOI:

https://doi.org/10.24193/subbbiol.2018.1.05

Keywords:

Dianthus, endemic/endangered species, genetic polymorphism, RAPD markers.

Abstract

Conservation of endangered and endemic species of Dianhtus from Romania, requires the investigation of genetic polymorphism in the populations. Preliminary data were obtained by molecular characterization using RAPD markers. DNA amplification with the 9 RAPD primers of the individuals belonging to different populations of D. callizonus, D. giganteus ssp. banaticus, D. glacialis ssp. gelidus, D. henteri, D. nardiformis, D. pratensis ssp. racovitzae, D. spiculifolius and D. tenuifolius revealed low level of polymorphism within and between populations. Several polymorphic RAPD markers were identified being useful for investigation of genetic diversity. Out the 9 primers studied by us, only the primer OPB-07 ensured amplification in all species and primers OPA-13, OPE-04 and 1225 showed positive results in most of the species. The primers 4A-26 and 4A-27 ensured amplification only in D. spiculifolius and the primers 4A-23 and OPM-18 gave no results in none of the species.

Author Biographies

Anca-Livia BUTIUC-KEUL, Babeş-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania. E-mail: anca.keul@ubbcluj.ro

Babeş-Bolyai University, Faculty of Biology and Geology, Department of Molecular Biology and Biotechnology, 400084 Cluj-Napoca, Romania.

Babeş-Bolyai University, Center of Systems Biology, Biodiversity and Bioresources, Laboratory of Plant Biology, Genetics, Microbiology and Biotechnology, 400006 Cluj-Napoca, Romania. E-mail: anca.keul@ubbcluj.ro

Liliana JARDA, Babeş-Bolyai University, Alexandru Borza Botanical Garden, Cluj-Napoca, Romania.

Babeş-Bolyai University, Alexandru Borza Botanical Garden, 400015 Cluj-Napoca, Romania. Email: liliana.jarda@ubbcluj.ro

Irina GOIA, Babeş-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania. Email:

Babeş-Bolyai University, Center of Systems Biology, Biodiversity and Bioresources, Laboratory of Plant Biology, Genetics, Microbiology and Biotechnology, 400006 Cluj-Napoca, Romania.

Babeş-Bolyai University, Faculty of Biology and Geology, Department of Taxonomy and Ecology,  400015 Cluj-Napoca, Romania. Email: irina.goia@ubbcluj.ro

Irina HOLOBIUC, Institute of Biology, Romanian Academy, Bucharest, Romania. Email: anca.keul@ubbcluj.ro

Institute of Biology, Romanian Academy, 060031 Bucharest, Romania. Email: anca.keul@ubbcluj.ro

Anca FARKAS, Babeş-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania. Email: anca.farkas@ubbcluj.ro

Babeş-Bolyai University, Faculty of Biology and Geology, Department of Molecular Biology and Biotechnology, 400084 Cluj-Napoca, Romania. Email: anca.farkas@ubbcluj.ro

 Babeş-Bolyai University, Center of Systems Biology, Biodiversity and Bioresources, Laboratory of Plant Biology, Genetics, Microbiology and Biotechnology, 400006 Cluj-Napoca, Romania.

Victoria CRISTEA, Babeş-Bolyai University, Alexandru Borza Botanical Garden, Cluj-Napoca, Romania. E-mail: anca.keul@ubbcluj.ro

Babeş-Bolyai University, Center of Systems Biology, Biodiversity and Bioresources, Laboratory of Plant Biology, Genetics, Microbiology and Biotechnology, 400006 Cluj-Napoca, Romania.

Babeş-Bolyai University, Alexandru Borza Botanical Garden, 400015 Cluj-Napoca, Romania.

E-mail: victoria.cristea@ubbcluj.ro

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Published

2018-06-22

How to Cite

BUTIUC-KEUL, A.-L., JARDA, L., GOIA, I., HOLOBIUC, I., FARKAS, A., & CRISTEA, V. (2018). Preliminary data regarding genetic diversity of several endangered and endemic “Dianthus” species from Romania generated by RAPD markers. Studia Universitatis Babeș-Bolyai Biologia, 63(1), 59–72. https://doi.org/10.24193/subbbiol.2018.1.05

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