Genetic Stability Assessment of “in Vitro” Plants by Molecular Markers
Keywords:
conservation, genetic fidelity, in vitro plants, micropropagation, molecular markers.Abstract
Genetic stability of in vitro plants should be assessed in order to develop proper programs of conservation. Such conservation programs could be developed only after evaluation of genetic variability in the natural populations and the genetic stability or somaclonal variability after conservation. In case of valuable economic plants as fruit trees, vegetables, medicinal plants, the natural variability from populations should be preserved as sources of new cultivars or variations. In order to preserve endangered, vulnerable or endemic plants it is very important to evaluate the genetic variability in natural populations and to preserve this variability as well as individuals or habitats. Genetic variability or stability after plant conservation is usually assessed by DNA-based molecular markers as RAPD, SSR, ISSR, SRAP, RFLP and AFLP.
References
Abe, T., Ii, N., Togashi, A., Sasahara, T. (2002) Large deletions in chloroplast DNA of rice calli after long-term culture, J. Plant Physiol., 159, 917-923
Alizadeh, M., Krishna, H., Eftekhari, M., Modareskia, M., Modareskia, M. (2015) Assessment of clonal fidelity in micropropagated horticultural plants, J. Chem. Pharm. Res., 7(12), 977-990
Aruja, S., Mandal, B. B., Dixit, S., Srivastava, P.S. (2002) Molecular, phenotypic and biosynthetic stability of Dioscorea floribunda plants derived from cryopreserved shoot tips, Plant Sci., 163, 971-977
Ateş Sönmezoğlu, Ö., Bozmaz, B., Yıldırım, A., Kandemir, N., Aydın, N. (2012) Genetic characterization of Turkish bread wheat landraces based on microsatellite markers and morphological characters, Turk. J. Biol., 36, 589-497
Barrett, C., Lefort, F., Douglas, G.C. (1997) Genetic characterization of oak seedlings, epicornic, crown and micropropagated shoots from mature tree by RAPD and microsatellite PCR, Sci. Hort., 70, 319-330
Bekheet, S.A., Gabr, A.M.M., Reda, A.A., El‐Bahr, M.K. (2015) Micropropagation and assessment of genetic satbility of in vitro raised jojoba (Simmondsia chinensis Link.) plant usind SCoT and ISSR markers, Plant Tiss. Cult. Biotech., 25(2), 165-179
Bhattacharyya, P., Kumaria, S., Diengdoh, R., Tandon, P. (2014) Genetic stability and phytochemical analysis of the in vitro regenerated plants of Dendrobium nobile Lindl., an endangered medicinal orchid, Meta Gene, 2, 489-504
Catană, R., Mitoi, M., Ion, R. (2013) The RAPD techniques used to assess the genetic diversity in Draba dorneri, a critically endangered plant species, Adv. Biosci. Bioteh., 4(2), 164-169
Chandrika, M., Thoyajaksha, V., Ravishankar Rai, V., Ramachandra Kini, K. (2008) Assessment of genetic stability of in vitro grown Dictyospermum ovalifolium, Biol. Plant., 52, 735-739
Condello, E., Palombi, M.A., Tonelli, M.G., Damiano, C., Caboni, E. (2009) Genetic stability of wild pear (Pyrus pyraster, Burgsd) after cryopreservation by encapsulation dehydration, Agr. Food Sci., 18, 136-143
Courtial, B., Feuerbach, F., Eberhard, S., Rohmer, S., Chiapello, H., Camilleri, C., Lucas, H. (2001) Tnt1 transposition events are induced by in vitro tranformation of Arabidopsis thaliana and transposed copies integrate into genes, Mol. Genet. Genomics, 265, 32-42
Cristea, V., Crăciunaş, C., Marcu, D., Palada, M., Butiuc-Keul, A. (2014) Genetic stability during in vitro propagation of Dianthus spiculifolius Schur., Prop. Ornam. Plants, 14(1), 26-31
Cuesta, C., Ordás, R.J., Rodríguez, A., Fernández, B. (2010) PCR-based molecular markers for assessment of somaclonal variation in Pinus pinea clones micropropagated in vitro, Biol. Plant., 54, 435-442
Deng, Z.N., Gentile, A., Nocolosi, E., Domina, F., Vardi, A., Tribulato, E. (1995) Identification of in vivo and in vitro mutants of lemon with RAPD markers, J. Hort. Sci., 70, 117-125
Devarumath, R.M., Nandy, S., Ravi, V., Marimuthu, S., Muralledharan, N, Raina, S.N. (2002) RAPD, ISSR and RFLP fingherprints as useful marker to evalute genetic integrity of micropropagated plants of three diploid and triploid elite tea clones representing Camelia sinensis (China type) and C. assamica ssp assamica (Assam-India type), Plant Cell Rep., 21, 166-173
Hao, Y-J., Wen, X-P., Deng, X-X. (2004) Genetic and epigenetic evaluations of citrus calluses recovered form slow-growth culture, J. Plant Physiol., 161, 479-484
Hashmi, G., Huettel, R., Meyer, R., Krusberg, L., Hammerschlag, F.A. (1997) RAPD analysis of somalonal variants derived from embryo callus cultures of peach, Plant Cell Rep., 16, 624-627
Hewezi, T., Jardinaud, F., Allibert, G., Kallerhoff, J. (2003) A new approach for efficient regeneration of a recalcitrant genotype of sunflower (Helianthus annuus) by organogenesis induction on split embryonic axes, Plant Cell, Tissue, Organ Cult., 73, 81-86
Hormaza, J.I. (1999) Early selection of cherry combining RAPDs with embryo culture, Sci. Hort., 79, 121-126
Jaligot, E., Beulé, T., Baurens F-C., Billotte, N., Rival, A. (2003) Search for methylation-sensitive ampification polymorphism associated with the “mantled’’ fenotype in oil palm (Elais gineensis Jask.), Genome, 47, 224-228
Jaligot, E., Beulé, T., Rival, A. (2002) Methylation-sensitive RFLPs: characterisation of two oil palm markers showing somaclonal variation-sensitive associated polymorphism, Theor. Appl. Genet., 104, 1263-1269
Jarda, L., Butiuc-Keul., A., Hőhn, M., Pedryc, A., Cristea, V. (2014) Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers, Turk. J. Biol., 38, 21-30
Joshi, P., Dhawan, V. (2007) Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay, Biol. Plant., 51, 22-26
Kantharajah, A.S., Golegaonkar, P.G. (2004) Somatic embryogenesis in eggplant, Sci. Hort., 99, 107-117
Kour, B., Kour, G., Kaul, S., Dhar, M.K. (2014) In vitro mass multiplication and assessment of genetic stability of in vitro raised Artemisia absinthium L. plants using ISSR and SSAP molecular markers, Adv. Bot., 1-7
Leroy, X.J., Leon, K., Charles, G., Branchard, M. (2000) Cauliflower somatic embryogenesis and analysis of regenerant stability by ISSRs, Plant Cell Rep., 19, 1102-1107
Modgil, M., Mahajan, K., Chakrabarti, S.K., Sharma, D.R., Sobti, R.C. (2005) Molecular analysis of genetic stability in micropropagated apple rootstock MM106, Sci. Hort., 104, 151-160
Mohanty, S., Panda, M.K., Sahoo, S., Nayak, S. (2011) Micropropagation of Zingiber rubens and assessment of genetic stability through RAPD and ISSR markers, Biol. Plant., 55, 16-20
Morcillo, F., Gabneur, C., Adam, H, Richaud, F., Singh, R., Cheah, S.C., Rival, A., Duval, Y., Tregear, J.W. (2006) Somaclonal variation in micropropagated oil palm. Characterizatin of two novel genes with enhanced expression in epigenetically abnormal cell lines and in response to auxin, Tree Physiol., 26, 585-594
Munthali, M.T., Newbury, H.J., Ford-Loyd, B.V. (1996) The detection of somaclonal variants of beet using RAPD, Plant Cell Rep., 15, 474-478
Palombi, M.A., Damiano, C. (2002) Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev), Plant Cell Rep., 20, 1061-1066
Peyvandi, M., Monsef, M., Hosseini-Mazinani, M. (2013) Assessment of genetic stability of olive in vitro propagated by RAPD marker, J. Cell Mol. Res., 5(2), 81-86
Rahman, M.H., Rajora, O.P. (2001) Microsatellite DNA somaclonal variation in micropropagated trembling aspen (Populus tremuloides), Plant Cell Rep., 20, 531-536
Rostiana, O., Riwa, M., Marubashi, W. (1999) Efficiency of inter-simple sequence repeat PCR for detecting somaclonal variation among leaf-culture-regenerated plants of horseradih, Breed. Sci., 49, 245-250
Saha, S., Adhikari, S., Dey, T., Ghosh, P. (2016) RAPD and ISSR based evaluation of genetic stability of micropropagated plantlets of Morus alba L. variety S-1, Meta Gene, 7, 7-15
Shu, Q.Y., Liu, G.S., Qi, D.M., Chu, C.C., Liu, J., Li, H.J. (2003) An effective method for axillary bud culture and RAPD analysis of cloned plants in tetraploid black locus, Plant Cell Rep., 22, 175-180
Soliman, H.I.A. (2013) Cryopreservation of in vitro-grown shoot tips of apricot (Prunus armeniaca L.) using encapsulation-dehydration, Afr. J. Biotechnol., 12(12), 1419-1430
Teixeira da Silva, J.A., Bolibok, H., Rakoczy-Trojanowska, M. (2007) Molecular markers in micropropagation, tissue culture and in vitro plant research, Genes, Genomes and Genomics, 1(1), 66-72
Tiwari, J.K., Chandel, P., Gupta, S., Gopal, J., Singh, BP., Bhardwaj, V. (2013) Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers, Physiol. Mol. Biol. Plants, 19(4), 587-595
Tudor, R.C. M. (2012) Assessment of genetic stability of plants obtained in vitro from Albizzia lebbeck and Albizzia julibrissin using RAPD markers, J. Hort. Forest. Biotechnol., 16(1), 225-231
Wallner, E., Weising, K., Rompf, R., Kahl, G., Kopp, B. (1996) Oligonucleotide fingerprinting and RAPD analysis of Achillea species: Characterization and long-term monitoring of micropropagated clones, Plant Cell Rep., 15, 647-652
Yi, J.Y., Chung, J.W., Lee, Y.Y., Kwak, J.G., Lee, S.Y. (2015) Morphological and genetic stability of dormant apple winter buds after cryopreservation, Korean J. Plant Res., 28(6), 697-703
Zietkiewicz, E., Rafalski, A., Labuda, D. (1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification, Genomics, 20, 176-183
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2016 Studia Universitatis Babeș-Bolyai Biologia
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.