Nitrate, nitrite and microbial denitrification in drinking water from Ozun village (Covasna County, Romania) and the association between changes during water storage
DOI:
https://doi.org/10.24193/subbbiol.2017.1.01Keywords:
denitrifying bacteria, groundwater, nitrate, nitrite, water storage.Abstract
Severe groundwater contamination affects drinking water quality in many vulnerable zones across Romania. Ingestion of high levels of nitrate and nitrite can lead to the emergence of compounds with major toxicological effects. The present study was aimed to assess nitrate and nitrite concentrations in parallel with the activity of denitrifying bacteria, in different groundwater sources from Ozun village, Covasna County. Natural denitrification potential based on local microbiota was investigated during water storage for one month. Results indicate that tap water supplied from the public system complies with drinking water criteria, but most domestic wells are not safe. Nitrate concentrations exceeded the maximum limit allowed for drinking water in most of the samples collected from dug wells, while nitrite ions occurred within the mandatory limit. Denitrifying bacteria were detected in all groundwater samples, with the exception of one well. Regrowth of denitrifying bacteria was observed during water storage, but significant reduction of nitrate, nitrite or their sum of ratio did not occurred as a general rule. The association between percentage changes in bacterial counts, nitrate and nitrite concentrations was not statistically significant. In conclusion, enhancing the bioremediation potential of local microbiota by groundwater storage at household level is not an efficient strategy for nitrate/ nitrite removal.
References
Burcă, S., Indolean, C., Măicăneanu, A. (2015) Groundwater quality in shallow wells from Feleacu village, Cluj County, Romania, Studia Universitatis Babeș-Bolyai, Chemia 60(3): 247-255
Butiuc-Keul, A. (2014) Biotehnologie Generală, Presa Universitară Clujeană, Cluj-Napoca, pp. 276 [in Romanian]
Council Directive 98/83/EC (1998) on the quality of water intended for human consumption, Official Journal of the European Communities, L330/32
Dahan, O., Babad, A., Lazarovitch, N., Russak, E. E., Kurtzman, D. (2014) Nitrate leaching from intensive organic farms to groundwater. Hydrology and Earth System Sciences 18: 333–341
Epure, L., Borda, D. R. (2014) Groundwater contamination and the relationship between water chemistry and biotic components in a karst system (Bihor Mountains, Romania), Travaux de l´Institut de Spéologie Emil Racovita 53: 69-84
Erisman, J. W., Galloway, J. N., Seitzinger, S., Bleeker, A., Dise, N. B., Petrescu, A. M. R., Leach, A. M., de Vries, W. (2013) Consequences of human modification of the global nitrogen cycle, Philosophical Transactions of the Royal Society B 20130116
Farkas, A. (2015) Apa potabilă și biofilmul, Presa Universitară Clujeană, Cluj-Napoca, pp. 198 [in Romanian]
Farkas, A., Drăgan-Bularda, M., Muntean, V., Ciatarâş, D., Ţigan Ş. (2013) Microbial activity in drinking water-associated biofilms, Central European Journal of Biology 8(2): 201-214
Fewtrell, L. (2004) Drinking water nitrate, methemoglobinemia, and global burden of disease: a discussion. Environ Health Perspectives 112(14): 1371–1374
Fields, S. (2004) Global nitrogen: cycling out of control. Environmental Health Perspectives 112(10): A556–A563
Fleşeriu, A., Oroian, I. (2010) Water pollution with nitrates from agricultural sources. ProEnvironment 3: 110 – 112
Grizzetti, B., Bouraoui, F., Billen, G., van Grinsven, H., Cardoso, A. C., Thieu, V., Garnier, J., Curtis, C., Howarth, R. W., Johnes, P. (2011) Nitrogen as a threat to European water quality. In: The European nitrogen assessment, Sutton, M. A., Howard, C. M., Erisman, J. W., Billen, G., Bleeker, A., Grennfelt, P., van Grinsven, H., Grizzetti, B., Cambridge University Press, Cambridge, New York, pp. 379-404
Hill, M. K. (2010) Understanding environmental pollution. Cambridge University Press, New York, pp. 585
Holloway, J. M., Dahlgren, R. A. (2002) Nitrogen in rock: Occurrences and biogeochemical implications, Global Biogeochemical Cycles 16(4): 65-1–65-17
Iacob, O., Tudor, A., Neamțu, A., Cristea, A. (2012) Apa de fântână: contaminarea cu nitrați și methemoglobinemia, Carol Davila, București, pp. 31 [in Romanian]
Kross, B. C. (2002) Nitrate toxicity and drinking water standards – a review, The Journal of Preventive Medicine 10(1): 3-10
Law 458 (2002): Legea nr. 458 din 8 iulie 2002 privind calitatea apei potabile, Monitorul Oficial, 552 [in Romanian]
Marinov, I., Marinov, A. M. (2014) A coupled mathematical model to predict the influence of nitrogen fertilization on crop, soil and groundwater quality, Water Resources Management 28(15): 5231–5246
Muntean, V. (2009) Microbiologie generală, Presa Universitară Clujeană, Cluj-Napoca, pp. 332 [in Romanian]
Mureșan, C., Farkas, A., Chakirou, C. (2011) Studiu asupra surselor proprii de apă – fântâni și izvoare – din județul Cluj, Romaqua 73: 38-42 [in Romanian]
Myers, S. S., Gaffikin, L., Golden, C. D., Ostfeld, R. S., Redford, K. H., Ricketts, T. H., Turner, W. R., Osofsky, S. A. (2013) Human health impacts of ecosystem alteration. Proceedings of the National Academy of Sciences 110(47): 18753–18760
Pele, M., Vasile, G., Artimon, M. (2010) Study regarding nitrogen pollutants in well waters from Romania. Scientific Papers, UASVM Bucharest, 53: 145-151
Ráduly, I. (2011) Strategia de dezvoltare locală a comunei Ozun, Alma Mater, Cluj-Napoca, pp. 126 [in Romanian]
Richard, A. M., Diaz, J. H., Kaye, A. D. (2014) Reexamining the risks of drinking water nitrates on public health. The Ochsner Journal 14: 392–398
Roşu, C., Roba, C., Piştea, I., Neş, M, Ozunu, A. (2014) Groundwater quality and its suitability for drinking and agricultural use in rural area from Cluj County (Floreşti Village), Scientific Papers Series Management, Economic Engineering in Agriculture and Rural Development 14(2): 247-252
SR EN ISO 26777 (2002) Calitatea apei. Determinarea conținutului de nitriți. Metoda prin spectrometrie de absorbție moleculară. Asociația de Standardizare din România [in Romanian]
SR ISO 7890-1 (1986) Calitatea apei. Determinarea conținutului de nitrați. Asociația de Standardizare din România [in Romanian]
Suk, W. A., Ahanchian, H., Asante, K. A., Carpenter, D. O., Diaz-Barriga, F., Ha, E. H., Huo, X., King, M., Ruchirawat, M., da Silva, E. R., Sly, L., Sly, P. D., Stein, R. T., van den Berg, M., Zar, H., Landrigan, P. J. (2016) Environmental pollution: an underrecognized threat to children’s health, especially in low- and middle-income countries, Environmental Health Perspectives 124(3): 41-45
Tociu, C., Marcu, E., Ciobotaru, I. E., Maria, C. (2016) Risk assessment of population exposure to nitrates/nitrites in groundwater: a case study approach. Journal of Environmental Research and Protection 13(3): 39-45
Török-Oance, R., Tulhină, D., Lazăr, M. (2013) Preliminary study on groundwater pollution with nitrites and nitrates in Gătaia (Timiș County), Annals of West University of Timişoara, Biology 16(2): 89-96
Vasilache, V., Filote, C., Cretu, M. A., Sandu, I., Coisin, V., Vasilache, T., Maxim, C. (2012) Monitoring of groundwater quality in some vulnerable areas in Botoşani County for nitrates and nitrites based pollutants, Environmental Engineering and Management Journal 11(2): 471-479
Vulpaşu, E., Racoviţeanu, G. (2016) Evaluation of the groundwater quality in Constanta County, seaside area. Energy Procedia 85: 612 – 619
Ward, M. H., deKok, T. M., Levallois, P., Brender, J., Gulis, G., Nolan, B. T., VanDerslice, J. (2005) Workgroup report: Drinking water nitrate and health. Recent findings and research needs. Environmental Health Perspectives 113(11): 1607-1614
WHO (2011) Guidelines for drinking-water quality, 4th edition, World Health Organization, Geneva, pp. 541
Wick, K., Heumesser, C., Schmid, E. (2012) Groundwater nitrate contamination: Factors and indicators, Journal of Environmental Management 111: 178-186
Zaiontz, C. (2015) Real statistics using Excel. www.real-statistics.com.
Zeman, C. L., Kross, B., Vlad, M. (2002) A nested case-control study of methemoglobinemia risk factors in children of Transylvania, Romania, Environmental Health Perspectives 110(8): 817-822
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