Plant growth response and nitrate reductase activities of roots of “Chromolaena odorata” in a model spent lubricating oil-polluted soil
DOI:
https://doi.org/10.24193/subbbiol.2017.2.09Keywords:
bioaccumulation, Chromolaena odorata, enzymes, nitrate assimilation, nitrate reductase, phytoremediation.Abstract
The ability of Chromolaena odorata propagated by stem cuttings and grown for 50 days in the soil containing five (5) different concentrations of spent lubricating oil (SLO) in soil (0, 1, 3, 6, 9 and 12 % SLO), was investigated. The experiments were watered daily at 70% moisture field capacity. Parameters such as number leaves per plant, shoot length, plant height as well as nitrate reductase activities were measured. Shoot length as well as leaf number were significantly (p < 0.05) reduced, compared to the control treatment. Results also showed that nitrate reductase activities increased slightly with time. However, beyond 40 days, nitrate reductase activity was not detected in 3% - 12% w/w oil-in-soil treatments, respectively. Pollution indices such as Contamination factor and Hazard Quotient, used in the present study indicated significant reduction in contamination values upon sowing of C. Odorata. Values obtained from Bioaccumulation Quotients also indicated that the plant was able to significantly bioaccumulate elements such as Fe, Cu and Ni present in the SLO-polluted soil.
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