Effect of microaerobiosis on photosystem II in “Synechococcus” sp. PCC 7002
Keywords:
Chlorophyll, Cyanobacterium, Fluorescence, Microoxic, psbA, RT-PCR.Abstract
Cyanobacterial oxygenic photosynthesis transformed the early Earth’s biosphere and resulted in complex aerobic life forms. During the course of evolution, cyanobacteria retained many genes that responded to specific environmental cues. Our study shows that microaerobic treatment did not significantly alter the functionality of photosystem II complex in Synechococcus sp. strain PCC 7002. Surprisingly, no changes in the induction levels of psbA genes, especially in D1’ isoform, were recorded. This finding is important as signals an atypical behaviour of D1’ isoform from Synechococcus sp. PCC 7002 to microaerobic stress as compared to other cyanobacterial strains.
References
Blankenship, R. E (1992) Origin and early evolution of photosynthesis, Photosynthesis Research 33, 91- 111
Chiș, C., Chiș, I., Sicora, O., Carmel, D., Grec, A., Ardelean, A., Dragoș, N., Sicora, C. (2014) Comparative analysis of D1 protein sequences in cyanobacteria, Studia UBB Biologia, 2, 5-17
Mulkidjanian, A. Y., Koonin, E. V., Makarova, K. S., Mekhedov, S. L., Sorokin, A., Wolf, Y. I., Dufresne, A., Partensky, F., Burd, H., Kaznadzey, D., Haselkorn, R., Galperin, M. Y. (2006) The cyanobacterial genome core and the origin of photosynthesis – Proc. Natl. Acad. Sci. U S A 103(35), 13126–13131
Mulo, P., Sicora, C., Aro, E. M. (2009) Cyanobacterial psbA gene family: optimization of oxygenic photosynthesis – Cell. Mol. Life Sci. 66(13), 3697-3710
Pakrasi, H. B., Riethman, H. C., Sherman, L. A. (1985) Organization of pigment proteins in the photosystem II complex of the cyanobacterium Anacystis nidulans R2, Proc. Natl. Acad. Sci. USA, 82, 6903– 6907
Rast, A., Heinz, S., Nickelsen, J. (2015) Biogenesis of thylakoid membranes, Biochim Biophys Acta 1847(9), 821–830
Sane, P. V., Ivanov, A. G., Sveshnikov, D. (2002) A transient exchange of the photosystem II reaction center protein D1:1 with D1:2 during low temperature stress of Synechococcus sp. PCC 7942 in the light lowers the redox potential of Q(B), J. Biol. Chem. 277, 32739–32745
Sicora, C. I., Appleton, S. E., Brown, C. M., Chung, J., Chandler, J., Cockshutt, A. M., Vass, I., Campbell, D. A. (2006) Cyanobacterial psbA families in Anabaena and Synechocystis encode trace, constitutive and UV B - induced D1 isoforms, Biochim Biophys Acta 1757(1), 47-56
Sicora, C. I., Brown, C. M., Cheregi O., Vass, I., Campbell, D. A. (2008) The psbA gene family responds differentially to light and UVB stress in Gloeobacter violaceus PCC 7421, a deeply divergent cyanobacterium, Biochim Biophys Acta 1777(2), 130-139
Sicora, C. I., Ho, F. M., Salminen, T., Styring, S., Aro, E. M. (2009) Transcription of a “silent” cyanobacterial psbA gene is induced by microaerobic conditions, Biochim. et Biophys. Acta 1787, 105–112
Stevens, S. E., Patterson, C. O. P., Myers, J. (1973) The production of hydrogen peroxide by blue-green algae: a survey, J. Phycol. 9, 427–430
Summerfield, T. C., Nagarajan, S., Sherman, L. A. (2011) Gene expression under low-oxygen conditions in the cyanobacterium Synechocystis sp. PCC 6803 demonstrates Hik31-dependent and independent responses, Microbiol. 157(2), 301-312
Summerfield, T. C., Toepel, J., Sherman L. A. (2008) Low-oxygen induction of normally cryptic psbA genes in cyanobacteria, Biochem. 47, 12939-12941
Trtílek, M., Kramer, D. M., Koblížek, M. (1997) Dual-modulation LED kinetic fluorometer, J. Lumin. 72, 597-599
Vinyard, D. J., Gimpel, J., Ananyev, G. M., Cornejo, M. A., Golden, S. S., Mayfield, S. P., Dismukes, S. P. (2013) Natural variants of photosystem II subunit D1 tune photochemical fitness to solar intensity, J. Biol. Chem. 288(8), 5451–5462
Voorhies, A. A., Biddanda, B. A., Kendall, S. T., Join, S., Marcus, D. N., Nold, S. C., Sheldon, N. D., Dick, G. J. (2012) Cyanobacterial life at low O2: community genomics and function reveal metabolic versatility and extremely low diversity in a Great Lakes sinkhole mat, Geobiology, 10: 250–267
Wegener, K. M., Nagarajan, A., Pakrasi, H. B. (2015) An atypical psbA gene encodes a sentinel D1 protein to form a physiologically relevant inactive photosystem II complex in cyanobacteria, J Biol. Chem. 290(6): 3764-3774
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2016 Studia Universitatis Babeș-Bolyai Biologia
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
