EXPLORING THE BIOLOGICAL PROTECTIVE ROLE OF CAROTENOIDS BY RAMAN SPECTROSCOPY: MECHANICAL STRESS OF CELLS

Authors

  • F. NEKVAPIL Biomolecular Physics Department, Babeş-Bolyai University, Str. Kogălniceanu 1, RO 400084 Cluj-Napoca, Romania; Physics of Nanostructured Systems Department, National Institute for Development of Isotopic and Molecular Technologies, Str. Donat 67-103, 400293 Cluj-Napoca, Romania.fran.nekvapil@itim-cj.ro https://orcid.org/0000-0001-8652-8292
  • CS. MÜLLER MOLNÁR Biomolecular Physics Department, Babeş-Bolyai University, Str. Kogălniceanu 1, RO 400084 Cluj-Napoca, Romania; Mass Spectrometry, Chromatography and Applied Physics Department, National Institute for Development of Isotopic and Molecular Technologies, Str. Donat 67-103, 400293 Cluj-Napoca, Romania. csilla.molnar@itim-cj.ro https://orcid.org/0000-0002-2677-3509
  • S. TOMŠIĆ Department of Aquaculture, University of Dubrovnik, Str. Ćira Carića str. 4, 20 000 Dubrovnik, Croatia. sanja.tomsic@unidu.hr https://orcid.org/0000-0001-9881-5475
  • S. CINTĂ PINZARU Biomolecular Physics Department, Babeş-Bolyai University, Str. Kogălniceanu 1, RO 400084 Cluj-Napoca, Romania. simona.cinta@phys.ubbcluj.ro https://orcid.org/0000-0001-8016-4408

DOI:

https://doi.org/10.24193/subbphys.2019.08

Keywords:

Raman spectroscopy, mechanical stress, sea urchin eggs, carotenoids

Abstract

Carotenoids present a group of tetraterpenoid biomolecules which are well known for their physiological benefits through their remarkable radical scavenging activity and reactive oxygen species quenching. However, little is known about role of carotenoids in relieving mechanical stress. Hence, in this study, we exposed sea urchin fertilized eggs to mechanical stress in form of centrifugation and exploited the advantage of Resonance Raman scattering to probe if the carotenoid profile or their distribution would change after application of the stress. Silver nanoparticles were used ro probe the Raman signal of carotenoids near the cell surface and attempt to achieve SERRS conditions (Surface-enhanced Resonance Raman scattering). We have found that carotenoid concentration notably increased on the cell surface after centrifugation, indicating that carotenoids were mobilized in response to mechanical stress.

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STUDIA UBB PHYSICA, Volume 64 (LXIV), No. 1-2, December 2019

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Published

2019-12-30

How to Cite

NEKVAPIL, F., MÜLLER MOLNÁR, C., TOMŠIĆ, S., & CINTĂ PINZARU, S. (2019). EXPLORING THE BIOLOGICAL PROTECTIVE ROLE OF CAROTENOIDS BY RAMAN SPECTROSCOPY: MECHANICAL STRESS OF CELLS. Studia Universitatis Babeș-Bolyai Physica, 64(1-2), 75–82. https://doi.org/10.24193/subbphys.2019.08

Issue

Volume 64, No. 1-2, 2019

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Articles

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