CYANOBACTERIA DETECTION AND RAMAN SPECTROSCOPY CHARACTERIZATION WITH A HIGHLY SENSITIVE, HIGH RESOLUTION FIBER OPTIC PORTABLE RAMAN SYSTEM

Authors

  • Simona CINTA PINZARU Babes-Bolyai University, Cluj-Napoca, Romania. corresponding author: simona.cinta@phys.ubbcluj.ro
  • Csilla MÜLLER Babes-Bolyai University, Cluj-Napoca, Romania. csilla.molnar@itim-cj.ro
  • Ioana BREZESTEAN Babes-Bolyai University, Cluj-Napoca, Romania. ioana.brezestean@itim-cj.ro https://orcid.org/0000-0001-6374-5155
  • Daniel BARCHEWITS B&W Tek Europe GmbH, Lübeck, Germany. corresponding author: simona.cinta@phys.ubbcluj.ro
  • Branko GLAMUZINA Department of Aquaculture, University of Dubrovnik, Croatia. corresponding author:  branko.glamuzina@unidu.hr https://orcid.org/0000-0002-5066-4599

Keywords:

cyanobacteria, Raman spectroscopy, portable Raman system, carotenoids.

Abstract

Raman spectroscopy of cultivated Synechocystis sp. AICB 51 strain cyanobacteria is reported both at single cell level and in bulk solution. Two types of equipment, a lab-based Raman system and a portable, high resolution fiber optic Raman system respectively, were employed, to characterize the bacteria and to assess the capability of the portable instrument to reproduce the lab results for environmental application purpose. Additional cyanobacteria isolated from the Adriatic Sea, probably Synechococcus (not yet genetically identified) was employed for Raman spectroscopy characterization. Both cyanobacteria strains showed characteristic bands at 1516, 1156 and 1006 cm-1, consistent with the three main Raman modes of beta-carotene in protein-carotenoid-complex from cyanobacteria, slightly different from those of pure beta-carotene. The portable system showed additional performance in recording higher signal to noise ratio in a faster and flexible way. A photoprotective energy dissipating mechanism involved upon laser exposure could be responsible for the similar Raman output of the two strains.

Author Biographies

Simona CINTA PINZARU, Babes-Bolyai University, Cluj-Napoca, Romania. corresponding author: simona.cinta@phys.ubbcluj.ro

Department of Biomolecular Physics, Babes-Bolyai University, Kogalniceanu 1, RO-400084, Cluj-Napoca, Romania. corresponding author: simona.cinta@phys.ubbcluj.ro

Csilla MÜLLER, Babes-Bolyai University, Cluj-Napoca, Romania. csilla.molnar@itim-cj.ro

Department of Biomolecular Physics, Babes-Bolyai University, Kogalniceanu 1, RO-400084, Cluj-Napoca, Romania. csilla.molnar@itim-cj.ro

Ioana BREZESTEAN, Babes-Bolyai University, Cluj-Napoca, Romania. ioana.brezestean@itim-cj.ro

Department of Biomolecular Physics, Babes-Bolyai University, Kogalniceanu 1, RO-400084, Cluj-Napoca, Romania. 

ioana.brezestean@itim-cj.ro

Daniel BARCHEWITS, B&W Tek Europe GmbH, Lübeck, Germany. corresponding author: simona.cinta@phys.ubbcluj.ro

B&W Tek Europe GmbH, Seelandstraße 14-16, 23569 Lübeck, Germany. corresponding author: simona.cinta@phys.ubbcluj.ro

Branko GLAMUZINA, Department of Aquaculture, University of Dubrovnik, Croatia. corresponding author:  branko.glamuzina@unidu.hr

Department of Aquaculture, University of Dubrovnik, Ćira Carića 4, 20000 Dubrovnik, Croatia.

corresponding author:  branko.glamuzina@unidu.hr

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STUDIA UBB PHYSICA, Volume 61 (LXI), No. 1, June, 2016

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Published

2016-06-30

How to Cite

CINTA PINZARU, S., MÜLLER, C., BREZESTEAN, I., BARCHEWITS, D., & GLAMUZINA, B. (2016). CYANOBACTERIA DETECTION AND RAMAN SPECTROSCOPY CHARACTERIZATION WITH A HIGHLY SENSITIVE, HIGH RESOLUTION FIBER OPTIC PORTABLE RAMAN SYSTEM. Studia Universitatis Babeș-Bolyai Physica, 61(1), 99–108. Retrieved from http://193.231.18.162/index.php/subbphysica/article/view/5820

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Volume 61, No. 1, 2016

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Articles

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