RAMAN IMAGING OF DENTAL FOLLICLE MESENCHYMAL STEM CELLS

Authors

  • Loredana F. LEOPOLD Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. loredana.leopold@usamvcluj.ro https://orcid.org/0000-0002-8084-1998
  • Cristina COMAN Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. cristina.coman@usamvcluj.ro https://orcid.org/0000-0002-7617-3763
  • Istvan Sz. TODOR Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca, Romania. istvan.todor88@gmail.com
  • Laszlo SZABO Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca, Romania. laszlo.szabo@phys.ubbcluj.ro https://orcid.org/0000-0002-7038-8317
  • Olga SORIȚĂU The Oncology Institute “Prof. Dr. Ion Chiricuţă”, Cluj-Napoca, Romania. corresponding author e-mail: vlad.moisoiu@gmail.com https://orcid.org/0000-0002-9270-8529
  • Piroska VIRAG The Oncology Institute “Prof. Dr. Ion Chiricuţă”, Cluj-Napoca, Romania. corresponding author e-mail: vpiroska@yahoo.com
  • Carmen M. MIHU Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. Corresponding author e-mail: carmenmihu2004@yahoo.com
  • Vlad MOISOIU Babeş-Bolyai University, Cluj-Napoca, Romania. Corresponding author e-mail: vlad.moisoiu@gmail.com
  • Nicolae LEOPOLD Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca, Romania. nicolae.leopold@ubbcluj.ro https://orcid.org/0000-0002-2174-8064

Keywords:

Raman mapping, principal component analysis, dental follicle mesenchymal stem cells, DNA, RNA.

Abstract

Raman spectroscopy is slowly but surely becoming an important investigative method in cellular samples. In this study, the potential of Raman mapping was assessed on dental follicle mesenchymal stem cells (MSCs). Raman spectra were recorded with the 532 nm laser line at ~1 mm spatial resolution. Principal Component Analysis (PCA) of the recorded Raman spectra provided false color Raman maps, evidencing cellular components such as the nucleus, nucleolus, endoplasmic reticulum and cytoplasmic RNA. A high concentration of this last component can be considered as a universal marker for stem cells. Given that stem cells are in a state where they are always ready to undergo transcription, a large concentration of RNA is found in the cytoplasm of these cells, as they rapidly need to produce proteins that specialize the cell during differentiation.

Author Biographies

Loredana F. LEOPOLD, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. loredana.leopold@usamvcluj.ro

Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Mănăştur 3-5, 400372 Cluj-Napoca, Romania. loredana.leopold@usamvcluj.ro; dana.leopold@gmail.com

Cristina COMAN, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. cristina.coman@usamvcluj.ro

Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Mănăştur 3-5, 400372 Cluj-Napoca, Romania. cristina.coman@usamvcluj.ro

Istvan Sz. TODOR, Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca, Romania. istvan.todor88@gmail.com

Faculty of Physics, Babeş-Bolyai University, Kogălniceanu 1, 400084 Cluj-Napoca, Romania. istvan.todor88@gmail.com

Laszlo SZABO, Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca, Romania. laszlo.szabo@phys.ubbcluj.ro

Faculty of Physics, Babeş-Bolyai University, Kogălniceanu 1, 400084 Cluj-Napoca, Romania. laszlo.szabo@phys.ubbcluj.ro

Olga SORIȚĂU, The Oncology Institute “Prof. Dr. Ion Chiricuţă”, Cluj-Napoca, Romania. corresponding author e-mail: vlad.moisoiu@gmail.com

Radiotherapy, Tumor and Radiobiology Laboratory, The Oncology Institute “Prof. Dr. Ion Chiricuţă”, Cluj-Napoca, Romania. corresponding author e-mail: vlad.moisoiu@gmail.com

Piroska VIRAG, The Oncology Institute “Prof. Dr. Ion Chiricuţă”, Cluj-Napoca, Romania. corresponding author e-mail: vpiroska@yahoo.com

Radiotherapy, Tumor and Radiobiology Laboratory, The Oncology Institute “Prof. Dr. Ion Chiricuţă”, Cluj-Napoca, Romania. corresponding author e-mail: vpiroska@yahoo.com

Carmen M. MIHU, Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. Corresponding author e-mail: carmenmihu2004@yahoo.com

Department of Histology, Iuliu Haţieganu University of Medicine and Pharmacy, Victor Babeş 8, 400012 Cluj-Napoca, Romania. Corresponding author e-mail: carmenmihu2004@yahoo.com

Vlad MOISOIU, Babeş-Bolyai University, Cluj-Napoca, Romania. Corresponding author e-mail: vlad.moisoiu@gmail.com

Faculty of Physics, Babeş-Bolyai University, Kogălniceanu 1, 400084 Cluj-Napoca, Romania.

Department of Histology, Iuliu Haţieganu University of Medicine and Pharmacy, Victor Babeş 8, 400012 Cluj-Napoca, Romania.

Corresponding author e-mail: vlad.moisoiu@gmail.com

Nicolae LEOPOLD, Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca, Romania. nicolae.leopold@ubbcluj.ro

Faculty of Physics, Babeş-Bolyai University, Kogălniceanu 1, 400084 Cluj-Napoca, Romania. nicolae.leopold@ubbcluj.ro

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STUDIA UBB PHYSICA, Volume 60 (LX), No. 2, December, 2015

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Published

2015-12-30

How to Cite

LEOPOLD, L. F., COMAN, C., TODOR, I. S., SZABO, L., SORIȚĂU, O., VIRAG, P., MIHU, C. M., MOISOIU, V., & LEOPOLD, N. (2015). RAMAN IMAGING OF DENTAL FOLLICLE MESENCHYMAL STEM CELLS. Studia Universitatis Babeș-Bolyai Physica, 60(2), 69–76. Retrieved from http://193.231.18.162/index.php/subbphysica/article/view/5940

Issue

Volume 60, No. 2, 2015

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Articles

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