Growth and development of salinity-exposed rice (“Oryza sativa”) rhizo-inoculated with “Bacillus subtilis” under different pH levels

Authors

DOI:

https://doi.org/10.24193/subbbiol.2019.2.04

Keywords:

Bacillus subtilis, FARO 44, Oryza sativa, pH, rice, salinity.

Abstract

The study investigated the effects of varying pH levels on the growth and development of salt-exposed rice (Oryza sativa L.) after inoculation with Bacillus subtilis. Germinated rice seedlings (var. FARO 44) were sown in garden soils amended to 100 mM NaCl, and were thereafter inoculated with Bacillus subtilis. The transplants were subsequently exposed to periodic wetting with 5 mL of pH-buffered water (pH 3, 5, 7, 9 and 11) every day, and with 5 mL of 100 mM NaCl every 4 days. The set up was monitored during a 30-day period. Results showed significant reduction in chlorophyll a and b as well as lycopene and tocopherol contents of leaves due to changes in the lipid-to-protein ratio of pigment-protein complex or increased chlorophyllase activity and drought stress. There were improved morphological characteristics such as plant height, sheath and foliar dimensions due to inoculation of B. subtilis. Increase in salinity resulted in a decrease in plant height, leaf length and sheath. Inoculation not only promoted rice growth, but also enhanced rice tolerance towards salinity owing to the fact that FARO 44 is a salinity-tolerant rice variety. A better understanding of the interaction between microbial inoculants and soil conditions is required to harness the desired benefits towards improving crop development.

Author Biographies

Francis Aibuedefe IGIEBOR, Wellspring University, Irhirhi, Benin City, Nigeria. E-mail: francis.igiebor@lifesci.uniben.edu

Department of Microbiology, Wellspring University, Irhirhi, Benin City, Nigeria; Environmental Biotechnology and Sustainability Research Group, Department of Plant Biology and Biotechnology, University of Benin, Nigeria. E-mail: francis.igiebor@lifesci.uniben.edu

Beckley IKHAJIAGBE, University of Benin, Nigeria. Email: francis.igiebor@lifesci.uniben.edu

Environmental Biotechnology and Sustainability Research Group, Department of Plant Biology and Biotechnology, University of Benin, Nigeria; Applied Environmental Biosciences and Public Health Research Group, Dept. of Microbiology, University of Benin, Nigeria. Email: francis.igiebor@lifesci.uniben.edu

Geoffrey Obinna ANOLIEFO, University of Benin, Nigeria. Email: francis.igiebor@lifesci.uniben.edu

Environmental Biotechnology and Sustainability Research Group, Department of Plant Biology and Biotechnology, University of Benin, Nigeria. Email: francis.igiebor@lifesci.uniben.edu

References

Aanchal, W., Amit, K.G., & Vatsala, S. (2019). Role of Bioactive Compounds in Human Health. Acta Scientific Medical Sciences, 3(9), 25-33.

Ahmadikhah, A., Nasrollanejad, S., & Alisha, O. (2008). Quantitative studies for investigating variation & its effect on heterosis of rice. International Journal of Plant Production, 2(4), 297 – 308

Ajala, A.S., & Gana, A. (2015). Analysis of Challenges Facing Rice Processing in Nigeria. Journal of Food Processing, 6, 893673.

Akinwale, M.G., Akinyele, B.O., Odiyi, A.C., Nwilene, F., Gregorio, G., & Oyetunji, O.E. (2012). Phenotypic screening of Nigerian rain-fed lowl & mega rice varieties for submergence tolerance. Proceedings of the World Congress on Engineering, July 4 – 6, London, pp. 640 – 648.

Anoliefo, G.O., Ikhajiagbe, B., Okoye, P.C., & Osayi, O. (2016). Utilizing local soap-derived biosurfactant for degradation of petroleum hydrocarbon polluted soils, sustainable remediation in focus. Annals of Science & Techonology, 1(1), 43 – 51.

Ashraf, M., & Harris, P.J.C. (2004). Potential biochemical indicators of salinity tolerance in plants. Plant Science, 166, 3–16.

Atkinson, N.J., Dew, T.P., Orfila, C., & Urwin, P.E. (2011). Influence of combined biotic & abiotic stress on nutritional quality parameters in tomato (Solanum lycopersicum L.). Journal of Agricultural & Food Chemistry, 59, 9673–9682.

Ayodele, O.K., Folasade, M.O., Joshua, O.A., & Chris O.A. (2014). Activity of the Antioxidant Defense System in a Typical Bioinsecticide-& Synthetic Insecticide-treated Cowpea Storage Beetle Callosobrochus maculatus F. (Coleoptera: Chrysomelidae). International Journal of Insect Science, 6, 99 –108.

Aziz, E.A., Al-Amier, H., & Craker, L.E. (2008a). Influence of Salt Stress on Growth & Essential Oil Production in Peppermint, Pennyroyal & Apple Mint. Journal of Herbs, Spices & Medicinal Plants, 14, 77 – 87.

Cha-Um, S., Supaibulwattana, K., & Kirdmanee, C. (2009). Comparative effects of salt stress & extreme pH stress combined on glycinebetaine accumulation, photosynthetic abilities & growth characters of two rice genotypes. Rice Science, 16, 274 – 282.

Chaves, M.M., Flexas, J., & Pinheiro, C. (2009). Photosynthesis under drought & salt stress: regulation mechanisms from whole plant to cell. Annals of Botany, 103, 551 – 560.

Chinnusamy, V., Jagendorf, A., & Zhu, J.K. (2005) Understanding & improving salt tolerance in plants. Crop Science, 45 (2), 437–448.

Dalton, T.J., & Guei, R.G. (2003) Productivity gains from rice genetic enhancements in West Africa: countries & ecologies. World Development, 31 (2), 359–374.

Das, P., Kamlesh, K.N., Sneh, L.S., & Pareek, A. (2015). Understanding salinty responses & adopting omics-based approaches to generate salinity tolerant cultivars of rice 6, 1 – 16.

Dogan, M., & Demirors, S.S. (2018). Pysiological effects of NaCl on Ceratophyllum demersum L., a submerged rootless aquatic macrophyte. Iranian Journal of Fisheries Sciences, 17(2), 346-356.

Favati, F., Lovelli, S., Galgano, F., Miccolis, V., Di Tommaso T., & Candido V. (2009). Processing tomato quality as affected by irrigation scheduling. Scientia Horticulturae, 122, 562–571.

Ghanem, M.E., Albacete, A., MartÍnez-Andújar, C., Acosta, M., Romero-Aranda, R., & Dodd, I. C. (2009). Hormonal changes during salinity-induced leaf senescence in tomato (Solanum lycopersicum L.). Journal of Experimental Botany, 59, 3039–3050.

Gholizadeh, F., & Navabpour, S. (2011). Effect of salinity on morphological and physiological characteristics in correlation to selection of salt tolerance in rice (Oryza sativa L.). International Journal of Agricultural Research, 6, 780-788.

Hayat, R., Ali, S., Amara, U., Khalid, R., & Ahmed, I. (2010). Soil beneficial bacteria & their role in plant growth promotion: a review. Annals of Microbiology, 60 (4), 579–598.

Ikhajiagbe, B., Anoliefo, G.O., Oshomoh, E.O., & Airhienbuwa, N. (2013). Changes in heavy metal contents of a waste engine oil polluted soil exposed to soil pH adjustments. British Biotechnology Journal, 3(2), 158-168.

Ikhajiagbe, B., Anoliefo, G.O., Oshomoh, E.O., Ogedegbe, U.A., & Airhienbuwa, N. (2012). Changes in polyaromatic hydrocarbon content of a waste engine oil polluted soil exposed to pH adjustments. Annual Review & Research in Biology, 2(3), 66-82.

Jamil, M., Lee, D.B., Jung, K.Y., Ashraf, M., Lee, S.C., & Rhal, E.S. (2006). Effects of salt (NaCl) stress on germination & early seedling growth of four vegetables species. Journal of Central European Agriculture, 7, 273–282.

Kapoor, N., & Pande, V. (2015). Effect of Salt Stress on Growth Parameters, Moisture Content, Relative Water Content & Photosynthetic Pigments of Fenugreek Variety RMt-1. Journal of Plant Sciences, 10, 210 – 221.

Lichtenthaler, H.K., & Buschmann, C. (2001). Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy, In: Current Protocols in Food Analytical Chemistry. New York, John Wiley & Sons, pp. 1–4.

Lichtenthaler, H.K. (1987). Chlorophylls & carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology, 148, 350 – 382.

Lugtenberg, B., & Kamilova, F. (2009). Plant-growth-promoting rhizobacteria. Annual Review of Microbiology, 63, 541–556.

Meloni, D.A., Olivia, M.A., Ruiz, H.A., & Martinez, C.A. (2001). Contribution of proline & inorganic solutes to osmotic adjustment in cotton under salt stress. Journal of Plant Nutrition, 24, 599 – 612.

Munns, R. (2003). Comparative Physiology of Salt & Water Stress. Plant, Cell & Environment, 25, 239 – 250.

Negrão, S., Schmöckel, S.M., & Tester, M. (2017). Evaluating physiological responses of plants to salinity stress. Annals of Botany, 119(1), 1–11.

Ojo. E.O., & Adebayo, P.F. (2012). Food security in Nigeria: an overview. European Journal of Sustainable Development, 1(2), 199 – 222

Paul, D., & Lade, H. (2014). Plant-growth-promoting rhizobacteria to improve crop growth in saline soils: a review. Agronomy for Sustainable Development, Springer Verlag/EDP Sciences/INRA, 34 (4), 737 – 752.

Prusty, M.R., Kim, S.R., & Vinarao, R. (2018). Newly Identified Wild Rice Accessions Conferring High Salt Tolerance Might Use a Tissue Tolerance Mechanism in Leaf. Front Plant Science, 9, 1 – 15

Riggi, E., Patanè, C., & Ruberto, G. (2008). Content of carotenoids a different ripening stage in processing tomato in relation to soil water availability. Australian Journal of Agricultural Research, 59, 348–353.

Sánchez-Rodríguez, E., Ruiz, J.M., Ferreres, F., & Moreno, D.A. (2012). Phenolic profiles of cherry tomatoes as influenced by hydric stress & rootstock technique. Food Chemistry, 134, 775–782.

Shabala, S.N., Shabala, S.I., Martynenko, A.I., Babourina, O., & Newman I.A. (1998). Salinity effect on bioelectric activity, growth, Na accumulation & chlorophyll fluorescence of maize leaves: a comparative survey & prospects for screening. Australian Journal of Plant Physiology, 25, 609–616

Shrivastava, P., & Kumar, R. (2015). Soil salinity: A serious environmental issue & plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences, 22, 123–131.

Udemezue, J.C. (2018). Analysis of Rice Production & Consumption Trends in Nigeria. Journal of Plant Sciences & Crop Protection, 1(3), 1 – 6.

Yadav, S.K. (2010). Heavy metal toxicity on plants: an overview on the note of gluthathione & phytochelatins in heavy metal the tolerance of plants. South African Journal of Botany, 76, 167 – 179.

Yang, F., Liang, Z.W., Wang, Z.E., & Chen, Y. (2008).Relationship between diurnal changes of net photosynthetic rate & influencing factors in rice under saline sodic stress. Rice Science, 15, 119 – 124.

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Published

2019-12-10

How to Cite

IGIEBOR, F. A., IKHAJIAGBE, B., & ANOLIEFO, G. O. (2019). Growth and development of salinity-exposed rice (“Oryza sativa”) rhizo-inoculated with “Bacillus subtilis” under different pH levels. Studia Universitatis Babeș-Bolyai Biologia, 64(2), 41–53. https://doi.org/10.24193/subbbiol.2019.2.04

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