Detection of beta-lactamase resistance genes in a hospital chlorinated wastewater treatment system
Keywords:
Antibiotic resistance genes, beta-lactam antibiotics, chlorination, hospital wastewater.Abstract
In this study, by amplification of several specific resistance genes we aimed at evaluating the presence of beta-lactamase resistance genes in the influent and effluent of a hospital chlorinated wastewater treatment system. Four types of beta-lactamase resistance genes (blaPER, blaVIM, blaNDM-1 and SHV) were detected in the influent water sample, two of which (blaVIM and SHV) being detected in the effluent as well. Our results indicate the reduced effectiveness of wastewater treatment, as several resistance genes can be found in the water discharged after the chlorination treatment.
References
De, A. S., Kumar, S. H., Baveja, S. M. (2010) Prevalence of metallo- β-lactamase producing Pseudomonas aeruginosa and Acinetobacter baumannii in intensive care areas in a tertiary care hospital, Indian J. Crit. Care Med., 14, 217-19
Gomes, E. S., Schuch, V., de Macedo Lemos, E. G. (2013) Biotechnology of polyketides: New breath of life for the novel antibiotic genetic pathways discovery through metagenomics, Braz. J. Microbiol., 44(4), 1007-1034
Hindiyeh, M., Smollen, G., Grossman, Z., Ram, D., Davidson, Y., Mileguir, F., Vax, M., David, B. D., Tal, I., Rahav, G., Shamiss, A., Mendelson, E., Keller, N. (2008) Rapid detection of blaKPCcarbapenemase genes by real-time PCR, J. Clinical Microbiol., 46(9), 2879-2883
Jia, S., Shi, P., Hu, Q., Li, B., Zhang, T., Zhang, X. X. (2015) Bacterial community shift drives antibiotic resistance promotion during drinking water chlorination, Environ. Sci. Technol. Article, ASAP DOI: 10.1021/acs.est.5b03521.
Jiang, L., Hu, X. L., Xu, T., Zhang, H. C., Sheng, D., Yin, D. Q. (2013) Prevalence of antibiotic resistance genes and their relationship with antibiotics in the Huangpu River and the drinking water sources, Shanghai, China, Sci. of the Total Environ., 458, 267-272
Naas, T., Ergani, A., Carred̈r, A., Nordmann, P. (2011) Real-time PCR for detection of NDM-1 carbapenemase genes from spiked stool samples, Antimicrobial Agents and Chemotherapy, 55(9), 4038-4043
Naas, T., Cotellon, G., Ergani, A., Nordmann, P. (2013) Real-time PCR for detection of blaOXA-48 genes from stools, J. of Antimicrob. Chemother., 68(1), 101-104
Poirel, L., Cabanne, L., Vahaboglu, H., Nordmann, P. (2005) Genetic environment and expression of the extended-spectrum β-lactamase blaPER-1 gene in gram-negative bacteria, Antimicrob. Agents Chemother., 49: 1708–1713
Pauwels, B., Verstraete, W. (2006) The treatment of hospital wastewater: an appraisal, J. Water and Health, 4(4), 405–416
Samaha-Kfoury, J. N., Araj, G. F. (2003) Recent developments in b-lactamases and extended spectrum b-lactamases, BMJ, 327, 1209–1213
Thomson, J. M., Bonomo, R. A. (2005) The threat of antibiotic resistance in Gram-negative pathogenic bacteria: beta-lactams in peril, Curr. Opin. Microbiol., 8(5), 518-524
Wan, M., Chou, C. (2015) Class 1 integrons and the antiseptic resistance gene (qaceδ1) in municipal and swine slaughterhouse wastewater treatment plants and wastewater—associated methicillin-resistant Staphylococcus aureus, Int. J. of Environ. Research and Public Health, 12(6), 6249-6260
Zhu, Y.-G., Johnson, T. A., Su, J.-Q., Qiao, M., Guo, G.-X., Stedtfeld, R. D., Hashsham, S. A., Tiedje, J. M. (2013) Diverse and abundant antibiotic resistance genes in Chinese swine farms, Proc. Natl. Acad. Sci. USA, 110(9), 3435-40
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